Reactivity of human antisera to codon optimized SARS-CoV2 viral proteins expressed in
COVID-19
Codon optimization
Escherichia coli expression system
N protein
SARS-CoV2 antisera
Journal
Tzu chi medical journal
ISSN: 2223-8956
Titre abrégé: Tzu Chi Med J
Pays: India
ID NLM: 101770275
Informations de publication
Date de publication:
Historique:
received:
25
07
2020
revised:
27
08
2020
accepted:
18
09
2020
entrez:
29
4
2021
pubmed:
30
4
2021
medline:
30
4
2021
Statut:
epublish
Résumé
The coronavirus disease 2019 (COVID-19) pandemic caused by the SARS-CoV2 virus continues to pose a serious threat to public health worldwide. The development of rapid diagnostic kits can assist the Tzu Chi Foundation in supporting global volunteers working to provide relief during the current pandemic. In this study, nucleotide sequences derived from publicly available viral genome data for several domains of the SARS-CoV2 spike and nucleocapsid (N) proteins were chemically synthesized, with codon optimization for N protein was expressed in amounts large enough to support large-scale production. The N-terminal domain, receptor-binding domain (RBD), Region 3, and the S2 domain were expressed in small but sufficient amounts for experiments. Immunoblotting results showed that anti-N IgG and anti-N IgM antibodies were detected in most patient sera, but only 60% of samples reacted with the recombinant RBD and S2 domain expressed by The results indicated that codon-optimized SARS-CoV2 viral proteins can be expressed in
Identifiants
pubmed: 33912411
doi: 10.4103/tcmj.tcmj_189_20
pii: TCMJ-33-146
pmc: PMC8059472
doi:
Types de publication
Journal Article
Langues
eng
Pagination
146-153Informations de copyright
Copyright: © 2021 Tzu Chi Medical Journal.
Déclaration de conflit d'intérêts
There are no conflicts of interest.
Références
Nat Med. 2020 Aug;26(8):1200-1204
pubmed: 32555424
J Virol Methods. 2020 Mar;277:113806
pubmed: 31911390
Protein Expr Purif. 2018 Apr;144:12-18
pubmed: 29180019
Biotechnol Bioeng. 2020 Jun;117(6):1684-1695
pubmed: 32086806
Curr Pharm Des. 2018;24(6):718-725
pubmed: 29384059
J Vet Sci. 2010 Jun;11(2):165-7
pubmed: 20458159
J Infect. 2020 May;80(5):554-562
pubmed: 32169481
Lancet. 2003 Dec 6;362(9399):1895-6
pubmed: 14667748
Protein Expr Purif. 1997 Oct;11(1):17-25
pubmed: 9325134
J Biosci Bioeng. 2015 Jan;119(1):19-27
pubmed: 25027708
J Infect Dis. 2004 Jul 15;190(2):379-86
pubmed: 15216476
J Virol. 2020 Jan 31;94(4):
pubmed: 31776274
Protein Expr Purif. 2013 Apr;88(2):235-42
pubmed: 23376461
J Biotechnol. 2008 Mar 20;134(1-2):64-71
pubmed: 18249455
Microbiol Res. 2018 Nov;216:30-39
pubmed: 30269854
Nucleic Acids Res. 1990 Mar 25;18(6):1465-73
pubmed: 2109307
Int J Mol Sci. 2019 Sep 09;20(18):
pubmed: 31505747
J Bacteriol. 1981 Mar;145(3):1365-73
pubmed: 7009583
Microbiol Resour Announc. 2020 Jun 11;9(24):
pubmed: 32527780
Cell Host Microbe. 2020 Mar 11;27(3):325-328
pubmed: 32035028
Int J Mol Sci. 2020 Jan 31;21(3):
pubmed: 32023820
Viruses. 2019 Jan 14;11(1):
pubmed: 30646565
Int J Biol Macromol. 2018 Jan;106:803-822
pubmed: 28830778
Infect Genet Evol. 2020 Apr;79:104212
pubmed: 32004758
Nature. 2020 May;581(7807):215-220
pubmed: 32225176
Nature. 2020 Jul;583(7816):459-468
pubmed: 32353859
J Infect. 2020 Jul;81(1):e28-e32
pubmed: 32283141