Urinary microRNAs as non-invasive biomarkers for toxic acute kidney injury in humans.

Acute Kidney Injury / chemically induced Animals Biomarkers / urine Glycine / analogs & derivatives Humans MicroRNAs / urine Oxalic Acid / toxicity Paraquat / poisoning Reproducibility of Results Daboia Viper Venoms / poisoning Glyphosate

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
28 04 2021
Historique:
received: 03 08 2020
accepted: 05 04 2021
entrez: 29 4 2021
pubmed: 30 4 2021
medline: 25 2 2023
Statut: epublish

Résumé

MicroRNAs in biofluids are potential biomarkers for detecting kidney and other organ injuries. We profiled microRNAs in urine samples from patients with Russell's viper envenoming or acute self-poisoning following paraquat, glyphosate, or oxalic acid [with and without acute kidney injury (AKI)] and on healthy controls. Discovery analysis profiled for 754 microRNAs using TaqMan OpenArray qPCR with three patients per group (12 samples in each toxic agent). From these, 53 microRNAs were selected and validated in a larger cohort of patients (Russell's viper envenoming = 53, paraquat = 51, glyphosate = 51, oxalic acid = 40) and 27 healthy controls. Urinary microRNAs had significantly higher expression in patients poisoned/envenomed by different nephrotoxic agents in both discovery and validation cohorts. Seven microRNAs discriminated severe AKI patients from no AKI for all four nephrotoxic agents. Four microRNAs (miR-30a-3p, miR-30a-5p, miR-92a, and miR-204) had > 17 fold change (p < 0.0001) and receiver operator characteristics area-under-curve (ROC-AUC) > 0.72. Pathway analysis of target mRNAs of these differentially expressed microRNAs showed association with the regulation of different nephrotoxic signaling pathways. In conclusion, human urinary microRNAs could identify toxic AKI early after acute injury. These urinary microRNAs have potential clinical application as early non-invasive diagnostic AKI biomarkers.

Identifiants

DOI: 10.1038/s41598-021-87918-0 PMID: 33911095 PMC: PMC8080685
pubmed: 33911095
doi: 10.1038/s41598-021-87918-0
pii: 10.1038/s41598-021-87918-0
pmc: PMC8080685
doi:

Substances chimiques

Biomarkers 0
MicroRNAs 0
Viper Venoms 0
Oxalic Acid 9E7R5L6H31
Paraquat PLG39H7695
Glycine TE7660XO1C

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

9165

Références

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Auteurs

Fathima Shihana (F)

Clinical Pharmacology and Toxicology Research Group, Biomedical Informatics and Digital Health, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW, Australia. fhan0023@sydney.edu.au.
South Asian Clinical Toxicology Research Collaboration, Faculty of Medicine, University of Peradeniya, Peradeniya, Sri Lanka. fhan0023@sydney.edu.au.
Centenary Institute of Cancer Medicine and Cell Biology, The University of Sydney, Sydney, NSW, Australia. fhan0023@sydney.edu.au.

Wilson K M Wong (WKM)

Diabetes and Islet Biology Group, School of Medicine, Western Sydney University, Campbelltown, NSW, Australia.

Mugdha V Joglekar (MV)

Diabetes and Islet Biology Group, School of Medicine, Western Sydney University, Campbelltown, NSW, Australia.

Fahim Mohamed (F)

Clinical Pharmacology and Toxicology Research Group, Biomedical Informatics and Digital Health, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW, Australia.
South Asian Clinical Toxicology Research Collaboration, Faculty of Medicine, University of Peradeniya, Peradeniya, Sri Lanka.
Allied Health Sciences, Department of Pharmacy, University of Peradeniya, Peradeniya, Sri Lanka.
Australian Kidney Biomarker Reference Laboratory, Department of Nephrology, Prince of Wales Hospital and Clinical School, University of New South Wales, Sydney, Australia.

Indika B Gawarammana (IB)

South Asian Clinical Toxicology Research Collaboration, Faculty of Medicine, University of Peradeniya, Peradeniya, Sri Lanka.

Geoffrey K Isbister (GK)

Clinical Toxicology Research Group, University of Newcastle, Newcastle, NSW, Australia.

Anandwardhan A Hardikar (AA)

Diabetes and Islet Biology Group, School of Medicine, Western Sydney University, Campbelltown, NSW, Australia.
Department of Science and Environment, Roskilde University, Roskilde, Denmark.

Devanshi Seth (D)

Centenary Institute of Cancer Medicine and Cell Biology, The University of Sydney, Sydney, NSW, Australia.
Discipline of Clinical Medicine and Addiction Medicine, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW, Australia.
Drug Health Services, Royal Prince Alfred Hospital, Sydney, NSW, Australia.

Nicholas A Buckley (NA)

Clinical Pharmacology and Toxicology Research Group, Biomedical Informatics and Digital Health, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW, Australia. nicholas.buckley@sydney.edu.au.
South Asian Clinical Toxicology Research Collaboration, Faculty of Medicine, University of Peradeniya, Peradeniya, Sri Lanka. nicholas.buckley@sydney.edu.au.
Drug Health Services, Royal Prince Alfred Hospital, Sydney, NSW, Australia. nicholas.buckley@sydney.edu.au.

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Classifications MeSH

Recherchemedicale.com › Maladies urogénitales › Maladies urologiques › Maladies du rein › Insuffisance rénale › Atteinte rénale aigüe › Urinary microRNAs as non-invasive biomarkers...
Recherchemedicale.com › Eucaryotes › Animaux › Urinary microRNAs as non-invasive biomarkers...
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Recherchemedicale.com › Acides aminés, peptides et protéines › Acides aminés › Glycine › Urinary microRNAs as non-invasive biomarkers...
Recherchemedicale.com › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Urinary microRNAs as non-invasive biomarkers...
Recherchemedicale.com › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN non traduit › Petit ARN non traduit › microARN › Urinary microRNAs as non-invasive biomarkers...
Recherchemedicale.com › Composés chimiques organiques › Acides carboxyliques › Acides acycliques › Diacides carboxyliques › Oxalates › Acide oxalique › Urinary microRNAs as non-invasive biomarkers...
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Recherchemedicale.com › Facteurs biologiques › Toxines biologiques › Venins › Venins de serpent › Venins de vipère › Urinary microRNAs as non-invasive biomarkers...