Nasal upregulation of
Journal
ERJ open research
ISSN: 2312-0541
Titre abrégé: ERJ Open Res
Pays: England
ID NLM: 101671641
Informations de publication
Date de publication:
Apr 2021
Apr 2021
Historique:
received:
07
12
2020
accepted:
27
01
2021
entrez:
26
4
2021
pubmed:
27
4
2021
medline:
27
4
2021
Statut:
epublish
Résumé
The clinical presentation of children sensitised to dog dander varies from asymptomatic to severe allergic airway disease, but the genetic mechanisms underlying these differences are not clear. The objective of the present study was to investigate nasal transcriptomic profiles associated with dog dander sensitisation in school children and to reveal clinical symptoms related with these profiles. RNA was extracted from nasal epithelial cell brushings of children sensitised to dog dander and healthy controls. Blood sample analyses included IgE against dog dander, dog allergen molecules, other airborne and food allergens, basophil activation and white blood cell counts. Clinical history of asthma and rhinitis was recorded, and lung function was assessed (spirometry, methacholine provocation and exhaled nitric oxide fraction). The most overexpressed gene in children sensitised to dog dander compared to healthy controls was Overexpression of
Sections du résumé
BACKGROUND
BACKGROUND
The clinical presentation of children sensitised to dog dander varies from asymptomatic to severe allergic airway disease, but the genetic mechanisms underlying these differences are not clear. The objective of the present study was to investigate nasal transcriptomic profiles associated with dog dander sensitisation in school children and to reveal clinical symptoms related with these profiles.
METHODS
METHODS
RNA was extracted from nasal epithelial cell brushings of children sensitised to dog dander and healthy controls. Blood sample analyses included IgE against dog dander, dog allergen molecules, other airborne and food allergens, basophil activation and white blood cell counts. Clinical history of asthma and rhinitis was recorded, and lung function was assessed (spirometry, methacholine provocation and exhaled nitric oxide fraction).
RESULTS
RESULTS
The most overexpressed gene in children sensitised to dog dander compared to healthy controls was
CONCLUSIONS
CONCLUSIONS
Overexpression of
Identifiants
pubmed: 33898616
doi: 10.1183/23120541.00917-2020
pii: 00917-2020
pmc: PMC8053908
pii:
doi:
Types de publication
Journal Article
Langues
eng
Informations de copyright
Copyright ©The authors 2021. This version is distributed under the terms of the Creative Commons Attribution Licence 4.0.
Déclaration de conflit d'intérêts
Conflict of interest: U. Käck reports a lecture fee from Thermo Fisher outside the submitted work. Conflict of interest: E. Einarsdottir has nothing to disclose. Conflict of interest: M. van Hage reports lecture fees from Thermo Fisher Scientific and ALK, and consultancy fees from Biomay AG, Vienna, Austria and Hycor Biomedical LLC, CA, US, outside the submitted work. Conflict of interest: A. Asarnoj has nothing to disclose. Conflict of interest: A. James has nothing to disclose. Conflict of interest: A. Nopp has nothing to disclose. Conflict of interest: K. Krjutškov has nothing to disclose. Conflict of interest: S. Katayama reports grants from the Jane and Aatos Erkko Foundation during the conduct of the study. Conflict of interest: J. Kere has nothing to disclose. Conflict of interest: G. Lilja has nothing to disclose. Conflict of interest: C. Söderhäll has nothing to disclose. Conflict of interest: J.R. Konradsen has received material from Thermo Fisher Scientific to perform IgE analysis in this project.
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