Phenotypically Anchored mRNA and miRNA Expression Profiling in Zebrafish Reveals Flame Retardant Chemical Toxicity Networks.

flame retardants (additives, reactives) gene expression mRNA micro-RNA (miRNA) network analysis neurodevelopment transcription factors (TFs) zebrafish

Journal

Frontiers in cell and developmental biology
ISSN: 2296-634X
Titre abrégé: Front Cell Dev Biol
Pays: Switzerland
ID NLM: 101630250

Informations de publication

Date de publication:
2021
Historique:
received: 02 02 2021
accepted: 03 03 2021
entrez: 26 4 2021
pubmed: 27 4 2021
medline: 27 4 2021
Statut: epublish

Résumé

The ubiquitous use of flame retardant chemicals (FRCs) in the manufacture of many consumer products leads to inevitable environmental releases and human exposures. Studying toxic effects of FRCs as a group is challenging since they widely differ in physicochemical properties. We previously used zebrafish as a model to screen 61 representative FRCs and showed that many induced behavioral and teratogenic effects, with aryl phosphates identified as the most active. In this study, we selected 10 FRCs belonging to diverse physicochemical classes and zebrafish toxicity profiles to identify the gene expression responses following exposures. For each FRC, we executed paired mRNA-micro-RNA (miR) sequencing, which enabled us to study mRNA expression patterns and investigate the role of miRs as posttranscriptional regulators of gene expression. We found widespread disruption of mRNA and miR expression across several FRCs. Neurodevelopment was a key disrupted biological process across multiple FRCs and was corroborated by behavioral deficits. Several mRNAs (e.g.,

Identifiants

DOI: 10.3389/fcell.2021.663032 PMID: 33898466 PMC: PMC8063052
pubmed: 33898466
doi: 10.3389/fcell.2021.663032
pmc: PMC8063052
doi:

Types de publication

Journal Article

Langues

eng

Pagination

663032

Subventions

Organisme : NIEHS NIH HHS
ID : P30 ES030287
Pays : United States
Organisme : NIEHS NIH HHS
ID : P42 ES016465
Pays : United States

Informations de copyright

Copyright © 2021 Dasgupta, Dunham, Truong, Simonich, Sullivan and Tanguay.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Auteurs

Subham Dasgupta (S)

The Sinnhuber Aquatic Research Laboratory, Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR, United States.

Cheryl L Dunham (CL)

The Sinnhuber Aquatic Research Laboratory, Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR, United States.

Lisa Truong (L)

The Sinnhuber Aquatic Research Laboratory, Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR, United States.

Michael T Simonich (MT)

The Sinnhuber Aquatic Research Laboratory, Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR, United States.

Christopher M Sullivan (CM)

Center for Genome Research and Computing, Oregon State University, Corvallis, OR, United States.

Robyn L Tanguay (RL)

The Sinnhuber Aquatic Research Laboratory, Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR, United States.

Classifications MeSH