Dynamic Pneumococcal Genetic Adaptations Support Bacterial Growth and Inflammation during Coinfection with Influenza.

Bacterial Proteins / genetics Coinfection Cytokines / metabolism Genetic Fitness Host-Pathogen Interactions / immunology Humans Inflammation Mediators Influenza A virus / immunology Influenza, Human / virology Leukocytes / immunology Mutation Pneumococcal Infections / immunology Streptococcus pneumoniae / physiology
genetic adaptation immune response influenza virus metabolism pathogenesis pneumococcus

Journal

Infection and immunity
ISSN: 1098-5522
Titre abrégé: Infect Immun
Pays: United States
ID NLM: 0246127

Informations de publication

Date de publication:
16 06 2021
Historique:
pubmed: 21 4 2021
medline: 21 9 2021
entrez: 20 4 2021
Statut: ppublish

Résumé

Streptococcus pneumoniae (pneumococcus) is one of the primary bacterial pathogens that complicates influenza virus infections. These bacterial coinfections increase influenza-associated morbidity and mortality through a number of immunological and viral-mediated mechanisms, but the specific bacterial genes that contribute to postinfluenza pathogenicity are not known. Here, we used genome-wide transposon mutagenesis (Tn-Seq) to reveal bacterial genes that confer improved fitness in influenza virus-infected hosts. The majority of the 32 genes identified are involved in bacterial metabolism, including nucleotide biosynthesis, amino acid biosynthesis, protein translation, and membrane transport. We generated mutants with single-gene deletions (SGD) of five of the genes identified, SPD1414, SPD2047 (

Identifiants

DOI: 10.1128/IAI.00023-21 PMID: 33875471 PMC: PMC8208518
pubmed: 33875471
pii: IAI.00023-21
doi: 10.1128/IAI.00023-21
pmc: PMC8208518
doi:

Substances chimiques

Bacterial Proteins 0
Cytokines 0
Inflammation Mediators 0

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

e0002321

Subventions

Organisme : NIAID NIH HHS
ID : R21 AI117247
Pays : United States
Organisme : NIAID NIH HHS
ID : U01 AI124302
Pays : United States
Organisme : NIAID NIH HHS
ID : R56 AI125324
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI139088
Pays : United States
Organisme : NCI NIH HHS
ID : R25 CA023944
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI110724
Pays : United States
Organisme : NIAID NIH HHS
ID : K25 AI100946
Pays : United States

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Auteurs

Amanda P Smith (AP)

Department of Pediatrics, University of Tennessee Health Science Center, Memphis, Tennessee, USA.

Lindey C Lane (LC)

Department of Pediatrics, University of Tennessee Health Science Center, Memphis, Tennessee, USA.

Tim van Opijnen (T)

Department of Biology, Boston College, Chestnut Hill, Massachusetts, USA.

Stacie Woolard (S)

Department of Flow Cytometry, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.

Robert Carter (R)

Department of Oncology, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.

Amy Iverson (A)

Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.

Corinna Burnham (C)

Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.

Peter Vogel (P)

Department of Pathology, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.

Dana Roeber (D)

The Hartwell Center, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.

Gabrielle Hochu (G)

Department of Pediatrics, University of Tennessee Health Science Center, Memphis, Tennessee, USA.

Michael D L Johnson (MDL)

Department of Immunobiology, University of Arizona, Tucson, Arizona, USA.

Jonathan A McCullers (JA)

Department of Pediatrics, University of Tennessee Health Science Center, Memphis, Tennessee, USA.

Jason Rosch (J)

Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.

Amber M Smith (AM)

Department of Pediatrics, University of Tennessee Health Science Center, Memphis, Tennessee, USA.

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Classifications MeSH

Recherchemedicale.com › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Dynamic Pneumococcal Genetic Adaptations...
Recherchemedicale.com › Infection › Co-infection › Dynamic Pneumococcal Genetic Adaptations...
Recherchemedicale.com › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Cytokines › Dynamic Pneumococcal Genetic Adaptations...
Recherchemedicale.com › Phénomènes génétiques › Aptitude génétique › Dynamic Pneumococcal Genetic Adaptations...
Recherchemedicale.com › Phénomènes biologiques › Interactions hôte-microbes › Interactions hôte-pathogène › Dynamic Pneumococcal Genetic Adaptations...
Recherchemedicale.com › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Dynamic Pneumococcal Genetic Adaptations...
Recherchemedicale.com › Facteurs biologiques › Médiateurs de l'inflammation › Dynamic Pneumococcal Genetic Adaptations...
Recherchemedicale.com › Virus › Virus à ARN › Virus à ARN de polarité négative › Orthomyxoviridae › Influenzavirus A › Virus de la grippe A › Dynamic Pneumococcal Genetic Adaptations...
Recherchemedicale.com › Maladies de l'appareil respiratoire › Infections de l'appareil respiratoire › Grippe humaine › Dynamic Pneumococcal Genetic Adaptations...
Recherchemedicale.com › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Dynamic Pneumococcal Genetic Adaptations...
Recherchemedicale.com › Phénomènes génétiques › Variation génétique › Mutation › Dynamic Pneumococcal Genetic Adaptations...
Recherchemedicale.com › Infection › Infections bactériennes et mycoses › Infections bactériennes › Infections bactériennes à Gram positif › Infections à streptocoques › Infections à pneumocoques › Dynamic Pneumococcal Genetic Adaptations...
Recherchemedicale.com › Bactéries › Bactéries à Gram positif › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus pneumoniae › Dynamic Pneumococcal Genetic Adaptations...