Assessment of Cytotoxic and Genotoxic Effect of Fissure Sealants in Buccal Epithelial Cells.
Biocompatibility
Buccal epithelial cells
Cytotoxins
Epithelial Cells
Fissure sealants
Materials Testing
MeSH terms: Pit and Fissure Sealants
Micronucleus assay
Mutagenicity Tests
Journal
Acta stomatologica Croatica
ISSN: 0001-7019
Titre abrégé: Acta Stomatol Croat
Pays: Croatia
ID NLM: 0253456
Informations de publication
Date de publication:
Mar 2021
Mar 2021
Historique:
entrez:
19
4
2021
pubmed:
20
4
2021
medline:
20
4
2021
Statut:
ppublish
Résumé
The main purpose of this study was to assess the genotoxic and cytotoxic effect of fissure sealants on buccal epithelial cells. The study was conducted on 45 patients (27 girls and 18 boys), seven to 16 years of age (age mean 12.09 ± 2.20). Buccal swabs were collected before (T0), seven (T1), 30 (T2) and 90 days (T3) consequently after fissure sealant placement (Helioseal F®, Equia Fil®, Constic®). Patients or legal guardians filled in the questionnaire regarding the demographic data (age, gender), dietary habits, health status, medication usage, and recent X-ray exposure. DNA damage was analyzed using the micronucleus test. Statistically significant difference in the number of buccal cells with condensed chromatin was found between T0 (time before fissure sealant placement) and T3 (90 days after fissure sealant placement) period for Helioseal F® (P = 0.025). For the other two analyzed materials, no difference was observed during the tested period. There was no difference between materials in the same sampling time. Apart from an increase in cells with condensed chromatin 90 days after the placement of Helioseal F®, no other nuclear abnormalities were observed for tested fissure sealants. Although these sealants have now largely been used, it is of high importance that their biocompatibility is checked continuously, especially in
Identifiants
pubmed: 33867533
doi: 10.15644/asc55/1/2
pii: ASC_55(1)_10-17
pmc: PMC8033626
doi:
Types de publication
Journal Article
Langues
eng
Pagination
10-17Déclaration de conflit d'intérêts
Conflict of interest: The authors declare no conflict of interest.
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