Molecular detection and characterization of Anaplasma platys and Ehrlichia canis in dogs from the Caribbean.
Anaplasma
/ enzymology
Anaplasmosis
/ epidemiology
Animals
Bacterial Proteins
/ analysis
Chaperonin 60
/ analysis
Citrate (si)-Synthase
/ analysis
Dog Diseases
/ epidemiology
Dogs
Ehrlichia canis
/ enzymology
Ehrlichiosis
/ epidemiology
Grenada
/ epidemiology
Prevalence
RNA, Bacterial
/ analysis
RNA, Ribosomal, 16S
/ analysis
RNA, Ribosomal, 23S
/ analysis
16S rRNA
23S rRNA
Anaplasma platys
Citrate synthase (gltA) and heat shock protein (groEL)
Ehrlichia canis
PCR
Tick-borne diseases
Journal
Ticks and tick-borne diseases
ISSN: 1877-9603
Titre abrégé: Ticks Tick Borne Dis
Pays: Netherlands
ID NLM: 101522599
Informations de publication
Date de publication:
07 2021
07 2021
Historique:
received:
06
10
2020
revised:
21
03
2021
accepted:
24
03
2021
pubmed:
18
4
2021
medline:
23
9
2021
entrez:
17
4
2021
Statut:
ppublish
Résumé
Anaplasma platys is a tick-transmitted rickettsial pathogen, which is known to be the etiologic agent for cyclic thrombocytopenia in its primary canine host. Infections with this pathogen are also reported in cats, cattle and people. Similarly, Ehrlichia canis is another tick-borne rickettsial pathogen responsible for canine monocytic ehrlichiosis and is also reported to cause infections in people. We describe infections in dogs with these two pathogens on the Caribbean island of Grenada, West Indies by detection using molecular methods. We utilized a 16S rRNA gene-based PCR assay to detect both Ehrlichia and Anaplasma species by screening 155 canine blood samples from asymptomatic dogs. We found 18.7 % of the dogs to be positive for A. platys and 16.8 % for E. canis. Samples that tested positive for A. platys were further assessed by sequence analysis targeting 16S rRNA, 23S rRNA, citrate synthase (gltA) and heat shock protein (groEL) genes. Phylogenetic analysis revealed high correlation of A. platys 16S rRNA and gltA gene sequences with the geographic origins, while 23S rRNA and groEL gene sequences clustered independent of the geographic origins. This study represents an important step in defining the widespread distribution of active rickettsial infections in Caribbean dogs with no apparent clinical signs, thus posing a high risk for canine health and to a lesser extent to humans, as most dogs in the Caribbean are free-roaming.
Identifiants
pubmed: 33865177
pii: S1877-959X(21)00080-7
doi: 10.1016/j.ttbdis.2021.101727
pmc: PMC8171494
mid: NIHMS1704376
pii:
doi:
Substances chimiques
Bacterial Proteins
0
Chaperonin 60
0
RNA, Bacterial
0
RNA, Ribosomal, 16S
0
RNA, Ribosomal, 23S
0
Citrate (si)-Synthase
EC 2.3.3.1
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
101727Subventions
Organisme : NIAID NIH HHS
ID : R01 AI070908
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI152418
Pays : United States
Organisme : NIAID NIH HHS
ID : R56 AI070908
Pays : United States
Informations de copyright
Copyright © 2021 Elsevier GmbH. All rights reserved.
Références
Acta Parasitol. 2014 Mar;60(1):21-5
pubmed: 26204016
Am J Vet Res. 1975 Jul;36(7):937-40
pubmed: 1147359
Vet Parasitol. 2014 Sep 15;205(1-2):285-8
pubmed: 25027609
Vector Borne Zoonotic Dis. 2010 Mar;10(2):117-23
pubmed: 19469667
Ticks Tick Borne Dis. 2014 Oct;5(6):943-6
pubmed: 25113982
J Feline Med Surg. 2014 Aug;16(8):713-20
pubmed: 24445821
J Mol Biol. 1990 Oct 5;215(3):403-10
pubmed: 2231712
Am J Vet Res. 1991 Sep;52(9):1537-41
pubmed: 1952347
Parasit Vectors. 2015 Feb 04;8:75
pubmed: 25649069
PLoS One. 2013;8(1):e53450
pubmed: 23335965
Mol Biol Evol. 2018 Jun 1;35(6):1547-1549
pubmed: 29722887
Pathogens. 2020 Apr 10;9(4):
pubmed: 32290349
J Clin Microbiol. 2007 Aug;45(8):2716-9
pubmed: 17596368
Ticks Tick Borne Dis. 2015 Sep;6(6):724-9
pubmed: 26100492
J Mol Evol. 1980 Dec;16(2):111-20
pubmed: 7463489
Parasit Vectors. 2013 Jul 23;6:213
pubmed: 23880226
Front Cell Infect Microbiol. 2017 Nov 29;7:490
pubmed: 29238699
MMWR Recomm Rep. 2016 May 13;65(2):1-44
pubmed: 27172113
Mol Biol Evol. 1992 Jul;9(4):678-87
pubmed: 1630306
Parasit Vectors. 2016 Mar 16;9:157
pubmed: 26984538
Vet Rec. 2009 Jan 10;164(2):59-61
pubmed: 19136688
Appl Environ Microbiol. 2002 Aug;68(8):3673-82
pubmed: 12147459
J Clin Microbiol. 1999 Aug;37(8):2631-8
pubmed: 10405413
Vector Borne Zoonotic Dis. 2013 Dec;13(12):846-50
pubmed: 24107211
Ann N Y Acad Sci. 2006 Oct;1078:110-7
pubmed: 17114689
BMC Vet Res. 2017 Nov 13;13(1):331
pubmed: 29132371
Vet Rec. 1997 Sep 6;141(10):247-50
pubmed: 9308149
Ticks Tick Borne Dis. 2012 Dec;3(5-6):288-93
pubmed: 23168049
Ticks Tick Borne Dis. 2016 Mar;7(2):276-83
pubmed: 26615872
Comp Immunol Microbiol Infect Dis. 2013 Mar;36(2):169-73
pubmed: 23273677
Rev Soc Bras Med Trop. 2006 Jan-Feb;39(1):64-7
pubmed: 16501769
Parasit Vectors. 2013 Apr 15;6:103
pubmed: 23587235
Acta Trop. 2020 Mar;203:105308
pubmed: 31862465
J Infect Dis. 1988 Jul;158(1):217-20
pubmed: 3392415
Int J Syst Evol Microbiol. 2001 Nov;51(Pt 6):2145-2165
pubmed: 11760958
Vector Borne Zoonotic Dis. 2020 Oct;20(10):755-762
pubmed: 32679008
Public Health Rep. 2011 May-Jun;126 Suppl 1:50-7
pubmed: 21563712
Parasit Vectors. 2010 Apr 08;3:26
pubmed: 20377860
N Engl J Med. 2005 Aug 11;353(6):587-94
pubmed: 16093467
J Vet Med Sci. 2017 Sep 12;79(9):1494-1502
pubmed: 28781347
J Vet Diagn Invest. 2017 Jan;29(1):109-114
pubmed: 27852813
Ann Trop Med Parasitol. 2003 Oct;97(7):769-72
pubmed: 14613636
Vector Borne Zoonotic Dis. 2015 Feb;15(2):170-2
pubmed: 25700049
Vet Parasitol. 2008 Feb 14;151(2-4):279-85
pubmed: 18160223
Am J Trop Med Hyg. 2010 Jul;83(1):174-82
pubmed: 20595498
Pathogens. 2020 Jun 19;9(6):
pubmed: 32575536
Vector Borne Zoonotic Dis. 2009 Feb;9(1):73-8
pubmed: 18847319
J Vet Intern Med. 2014 Nov-Dec;28(6):1702-7
pubmed: 25274547
Lancet Infect Dis. 2017 Jun;17(6):e189-e196
pubmed: 28365226
Vet J. 2011 Mar;187(3):292-6
pubmed: 20226700
Braz J Microbiol. 2010 Apr;41(2):381-5
pubmed: 24031508
Nature. 2006 Mar 30;440(7084):605
pubmed: 16572146
Rev Bras Parasitol Vet. 2011 Jan-Mar;20(1):1-12
pubmed: 21439224
Front Vet Sci. 2019 Jun 04;6:164
pubmed: 31214605
Am J Trop Med Hyg. 2014 Dec;91(6):1161-5
pubmed: 25266347
J Clin Microbiol. 1996 Sep;34(9):2133-9
pubmed: 8862572
Vet Parasitol. 2013 Sep 1;196(1-2):44-9
pubmed: 23481028
J Med Entomol. 1990 Nov;27(6):1065-6
pubmed: 2280392
Ticks Tick Borne Dis. 2020 May;11(3):101367
pubmed: 31987818
Trans R Soc Trop Med Hyg. 2000 Nov-Dec;94(6):707-8
pubmed: 11198664
Ann Agric Environ Med. 2003;10(2):137-41
pubmed: 14677903
Ticks Tick Borne Dis. 2012 Dec;3(5-6):332-7
pubmed: 23182271
Comp Immunol Microbiol Infect Dis. 2015 Apr;39:39-45
pubmed: 25748051
Ticks Tick Borne Dis. 2020 Nov;11(6):101517
pubmed: 32993937
Parassitologia. 1999 Dec;41(4):555-60
pubmed: 10870560
An Acad Bras Cienc. 2017 Jan-Mar;89(1):301-306
pubmed: 28423085
Exp Appl Acarol. 2018 Aug;75(4):429-435
pubmed: 30073430
Ann Trop Med Parasitol. 1991 Oct;85(5):539-42
pubmed: 1809248
Res Vet Sci. 2011 Aug;91(1):95-7
pubmed: 20723954
J Med Entomol. 2011 Mar;48(2):418-21
pubmed: 21485383
Parasit Vectors. 2015 Oct 06;8:506
pubmed: 26438311
Parasit Vectors. 2018 Nov 12;11(1):585
pubmed: 30419951
J Clin Microbiol. 2000 Nov;38(11):4219-21
pubmed: 11060094
J Infect Dis. 1978 Feb;137(2):182-8
pubmed: 627738
Rev Inst Med Trop Sao Paulo. 2016;58:35
pubmed: 27074329
Clin Diagn Lab Immunol. 2002 Sep;9(5):1132-6
pubmed: 12204973
Vet Ophthalmol. 2007 May-Jun;10(3):137-42
pubmed: 17445073
Vector Borne Zoonotic Dis. 2006 Winter;6(4):423-9
pubmed: 17187578
Ann N Y Acad Sci. 2006 Oct;1078:338-41
pubmed: 17114735
Am J Vet Res. 1977 Dec;38(12):1953-5
pubmed: 596693
JAMA. 1987 Jun 12;257(22):3100-4
pubmed: 3586228
Vet Parasitol. 2016 Jul 15;224:7-12
pubmed: 27270383
Vet J. 2010 Feb;183(2):232-3
pubmed: 19056304
Clin Diagn Lab Immunol. 2001 Mar;8(2):241-4
pubmed: 11238202
Parasit Vectors. 2010 Apr 08;3(1):33
pubmed: 20377870
Ticks Tick Borne Dis. 2016 Jul;7(5):938-944
pubmed: 27132516