Herpes Simplex Virus 1 Coinfection Modifies Adeno-associated Virus Genome End Recombination.
Animals
Cell Line
Chlorocebus aethiops
Coinfection
/ pathology
Dependovirus
/ genetics
Genome, Viral
/ genetics
HEK293 Cells
HeLa Cells
Helper Viruses
/ genetics
Herpes Simplex
/ pathology
Herpesvirus 1, Human
/ genetics
High-Throughput Nucleotide Sequencing
Humans
Parvoviridae Infections
/ pathology
Recombination, Genetic
/ genetics
Terminal Repeat Sequences
/ genetics
Vero Cells
Viral Interference
/ genetics
Virus Replication
/ genetics
AAV
HSV-1
adeno-associated virus
genome end recombination
herpes simplex virus type 1
Journal
Journal of virology
ISSN: 1098-5514
Titre abrégé: J Virol
Pays: United States
ID NLM: 0113724
Informations de publication
Date de publication:
10 06 2021
10 06 2021
Historique:
pubmed:
16
4
2021
medline:
26
8
2021
entrez:
15
4
2021
Statut:
ppublish
Résumé
Wild-type adeno-associated virus (AAV) can only replicate in the presence of helper factors, which can be provided by coinfecting helper viruses such as adenoviruses and herpesviruses. The AAV genome consists of a linear, single-stranded DNA (ssDNA), which is converted into different molecular structures within the host cell. Using high-throughput sequencing, we found that herpes simplex virus 1 (HSV-1) coinfection leads to a shift in the type of AAV genome end recombination. In particular, open-end inverted terminal repeat (ITR) recombination was enhanced, whereas open-closed ITR recombination was reduced in the presence of HSV-1. We demonstrate that the HSV-1 protein ICP8 plays an essential role in HSV-1-mediated interference with AAV genome end recombination, indicating that the previously described ICP8-driven mechanism of HSV-1 genome recombination may be underlying the observed changes. We also provide evidence that additional factors, such as products of true late genes, are involved. Although HSV-1 coinfection significantly changed the type of AAV genome end recombination, no significant change in the amount of circular AAV genomes was identified.
Identifiants
pubmed: 33853961
pii: JVI.00486-21
doi: 10.1128/JVI.00486-21
pmc: PMC8315985
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0048621Subventions
Organisme : Medical Research Council
ID : MR/N022890/1
Pays : United Kingdom
Organisme : Swiss National Science Foundation
ID : P1ZHP3_174912
Pays : Switzerland
Organisme : Swiss National Science Foundation
ID : 310030_184766/1
Pays : Switzerland
Organisme : UKRI | Medical Research Council (MRC)
ID : MR/N022890/1
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