KIFC1 regulates ZWINT to promote tumor progression and spheroid formation in colorectal cancer.
Antineoplastic Agents
/ pharmacology
Biomarkers, Tumor
/ metabolism
Cell Line, Tumor
Cell Proliferation
Colorectal Neoplasms
/ drug therapy
Diterpenes
/ pharmacology
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Humans
Immunohistochemistry
Intracellular Signaling Peptides and Proteins
/ metabolism
Kinesins
/ genetics
Nuclear Proteins
/ metabolism
RNA, Small Interfering
Transfection
KIFC1
ZWINT
colorectal cancer
kolavenic acid analog
Journal
Pathology international
ISSN: 1440-1827
Titre abrégé: Pathol Int
Pays: Australia
ID NLM: 9431380
Informations de publication
Date de publication:
Jul 2021
Jul 2021
Historique:
received:
12
01
2021
accepted:
12
03
2021
pubmed:
6
4
2021
medline:
19
1
2022
entrez:
5
4
2021
Statut:
ppublish
Résumé
Colorectal cancer (CRC) is the second leading cause of cancer-related mortality worldwide. Kinesin Family Member C1 (KIFC1) has been proposed as a promising therapeutic target due to its pivotal role in centrosome clustering to mediate cancer cell progression. This study aimed to analyze the expression and biological function of KIFC1 in CRC. Immunohistochemically, 67 (52%) of 129 CRC cases were positive for KIFC1 and statistically associated with poorer overall survival. KIFC1 small interfering RNA (siRNA)-transfected cells demonstrated lower cell proliferation as compared to the negative control cells. A specific KIFC1 inhibitor, kolavenic acid analog (KAA) drastically inhibited CRC cell proliferation. Microarray analysis revealed that KAA-treated CRC cells presented reduced ZW10 interacting kinetochore protein (ZWINT) expression as compared to control cells. Immunohistochemical analysis demonstrated that 61 (47%) of 129 CRC cases were positive for ZWINT and ZWINT expression was significantly correlated with KIFC1 expression. ZWINT-positive cases exhibited significantly worse overall survival. KIFC1 siRNA-transfected cells showed reduced ZWINT expression while ZWINT siRNA-transfected cells decreased cell proliferation. Both KIFC1 and ZWINT knockdown cells attenuated spheroid formation ability. This study provides new insights into KIFC1 regulating ZWINT in CRC progression and its potential as a therapeutic target.
Substances chimiques
Antineoplastic Agents
0
Biomarkers, Tumor
0
Diterpenes
0
Intracellular Signaling Peptides and Proteins
0
KIFC1 protein, human
0
Nuclear Proteins
0
RNA, Small Interfering
0
ZWINT protein, human
0
kolavenol
0
Kinesins
EC 3.6.4.4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
441-452Subventions
Organisme : Takeda Science Foundation
Organisme : Japan Society for the Promotion of Science
Informations de copyright
© 2021 Japanese Society of Pathology and John Wiley & Sons Australia, Ltd.
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