Engineered Cells as a Test Platform for Radiohaptens in Pretargeted Imaging and Radioimmunotherapy Applications.


Journal

Bioconjugate chemistry
ISSN: 1520-4812
Titre abrégé: Bioconjug Chem
Pays: United States
ID NLM: 9010319

Informations de publication

Date de publication:
21 04 2021
Historique:
pubmed: 6 4 2021
medline: 15 9 2021
entrez: 5 4 2021
Statut: ppublish

Résumé

Pretargeted imaging and radioimmunotherapy approaches are designed to have superior targeting properties over directly targeted antibodies but impose more complex pharmacology, which hinders efforts to optimize the ligands prior to human applications. Human embryonic kidney 293T cells expressing the humanized single-chain variable fragment (scFv) C825 (huC825) with high-affinity for DOTA-haptens (293T-huC825) in a transmembrane-anchored format eliminated the requirement to use other pretargeting reagents and provided a simplified, accelerated assay of radiohapten capture while offering normalized cell surface expression of the molecular target of interest. Using binding assays,

Identifiants

pubmed: 33819023
doi: 10.1021/acs.bioconjchem.0c00595
pmc: PMC8284561
mid: NIHMS1715935
doi:

Substances chimiques

Haptens 0
Radiopharmaceuticals 0

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

649-654

Subventions

Organisme : NCI NIH HHS
ID : R01 CA055349
Pays : United States
Organisme : NCI NIH HHS
ID : R35 CA241894
Pays : United States
Organisme : NCI NIH HHS
ID : P01 CA023766
Pays : United States
Organisme : NIBIB NIH HHS
ID : F32 EB025050
Pays : United States
Organisme : NCI NIH HHS
ID : P50 CA192937
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA008748
Pays : United States
Organisme : NCI NIH HHS
ID : F31 CA239511
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR002384
Pays : United States
Organisme : NCI NIH HHS
ID : K12 CA184746
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA233896
Pays : United States
Organisme : NCI NIH HHS
ID : R50 CA243895
Pays : United States

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Auteurs

Megan M Dacek (MM)

Molecular Pharmacology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10065, United States.
Department of Pharmacology, Weill Cornell Medical College, New York, New York 10065, United States.

Darren R Veach (DR)

Molecular Imaging and Therapy Service, Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, New York 10065, United States.
Radiochemistry and Imaging Sciences Service, Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, New York 10065, United States.
Department of Radiology, Weill Cornell Medical College, New York, New York 10065, United States.

Sarah M Cheal (SM)

Molecular Pharmacology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10065, United States.

Lukas M Carter (LM)

Department of Medical Physics, Memorial Sloan Kettering Cancer Center, New York, New York 10065, United States.

Michael R McDevitt (MR)

Molecular Imaging and Therapy Service, Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, New York 10065, United States.
Department of Radiology, Weill Cornell Medical College, New York, New York 10065, United States.

Blesida Punzalan (B)

Molecular Pharmacology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10065, United States.

Daniela Burnes Vargas (D)

Molecular Pharmacology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10065, United States.

Thomas Z Kubik (TZ)

Molecular Imaging and Therapy Service, Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, New York 10065, United States.

Sebastien Monette (S)

Tri-Institutional Laboratory of Comparative Pathology, Memorial Sloan Kettering Cancer Center, Weill Cornell Medicine, and The Rockefeller University, New York, New York 10065, United States.

Brian H Santich (BH)

Department of Pediatrics, Memorial Sloan Kettering Cancer Center, New York, New York 10065, United States.

Guangbin Yang (G)

Organic Synthesis Core Facility, Memorial Sloan Kettering Cancer Center, New York, New York 10065, United States.

Ouathek Ouerfelli (O)

Organic Synthesis Core Facility, Memorial Sloan Kettering Cancer Center, New York, New York 10065, United States.

Adam L Kesner (AL)

Department of Medical Physics, Memorial Sloan Kettering Cancer Center, New York, New York 10065, United States.

Nai-Kong V Cheung (NV)

Department of Pediatrics, Memorial Sloan Kettering Cancer Center, New York, New York 10065, United States.

David A Scheinberg (DA)

Molecular Pharmacology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10065, United States.
Department of Pharmacology, Weill Cornell Medical College, New York, New York 10065, United States.

Steven M Larson (SM)

Molecular Pharmacology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10065, United States.
Molecular Imaging and Therapy Service, Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, New York 10065, United States.
Department of Radiology, Weill Cornell Medical College, New York, New York 10065, United States.

Simone Krebs (S)

Molecular Imaging and Therapy Service, Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, New York 10065, United States.
Department of Radiology, Weill Cornell Medical College, New York, New York 10065, United States.

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Classifications MeSH