A 'Dilute and Shoot' Liquid Chromatography-Mass Spectrometry Method for Multiclass Drug Analysis in Pre-Cut Dried Blood Spots.
UPLC-MS/MS
dilute and shoot
ototoxic drugs
pre-cut dried blood spots
sleep inducers
whole blood analysis
work safety
Journal
International journal of environmental research and public health
ISSN: 1660-4601
Titre abrégé: Int J Environ Res Public Health
Pays: Switzerland
ID NLM: 101238455
Informations de publication
Date de publication:
16 03 2021
16 03 2021
Historique:
received:
04
02
2021
revised:
12
03
2021
accepted:
13
03
2021
entrez:
3
4
2021
pubmed:
4
4
2021
medline:
24
4
2021
Statut:
epublish
Résumé
Drugs able to affect the auditory and nervous systems and consumed by workers to treatdifferent pathologies can represent a possible source of risk in the work environment. All the target compounds involved in the presented project show ototoxic and/or narcoleptic side effects and, for these reasons, occupational safety organizations have recognized them as potential causes of work injuries. A multiclass method for the analysis of 15 drugs among the most widespread worldwide (belonging to nine different classes including antihistamines, beta-blockers, antidepressants, Z-drugs and opioids), was developed and validated. This study describes a rapid, sensitive and effective method to analyse these substances in whole blood using tailored pre-cut dried blood spots. Detection was achieved with a triple quadrupole mass spectrometer after an easy and simple 'dilute and shoot' solubilisation followed by an UPLC separation. All the issues linked to the use of the dried blood spots and whole blood, such as haematocrit variability, volumetric evaluation and sample carrier choice were carefully studied and managed during method development. From the validation study results it emerged that this approach can be deemed successful thanks to its few pg µL
Identifiants
pubmed: 33809736
pii: ijerph18063068
doi: 10.3390/ijerph18063068
pmc: PMC8002310
pii:
doi:
Substances chimiques
Pharmaceutical Preparations
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Références
Ther Drug Monit. 2007 Aug;29(4):447-51
pubmed: 17667799
Anal Bioanal Chem. 2017 May;409(13):3383-3392
pubmed: 28299418
J Pharm Pharmacol. 2012 May;64(5):705-11
pubmed: 22471366
J Chromatogr A. 2016 Jun 17;1451:41-49
pubmed: 27180888
Talanta. 2018 May 15;182:218-224
pubmed: 29501143
Clin Pharmacol Ther. 2009 Jun;85(6):596-9
pubmed: 19279562
J Anal Toxicol. 2012 Mar;36(2):106-11
pubmed: 22337779
Eur Rev Med Pharmacol Sci. 2011 Jun;15(6):601-36
pubmed: 21796866
Ther Drug Monit. 1990 May;12(3):264-7
pubmed: 2349610
Drug Test Anal. 2015 Apr;7(4):336-40
pubmed: 25565078
J Pharm Biomed Anal. 2019 Jan 30;163:188-196
pubmed: 30317075
Mass Spectrom Rev. 2016 May-Jun;35(3):361-438
pubmed: 25252132
Bioanalysis. 2018 Jun 1;10(11):839-850
pubmed: 29863895
J Clin Pharmacol. 1986 Sep-Oct;26(7):529-33
pubmed: 3760245
Bioanalysis. 2010 Aug;2(8):1385-95
pubmed: 21083339
Ther Drug Monit. 2019 Aug;41(4):409-430
pubmed: 31268966
Clin Biochem. 2016 Sep;49(13-14):1035-46
pubmed: 27179588
Pediatrics. 1963 Sep;32:338-43
pubmed: 14063511
J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Sep 15;1095:32-38
pubmed: 30041087
Bioanalysis. 2011 Oct;3(20):2321-33
pubmed: 22011180
N Engl J Med. 2015 Jan 15;372(3):241-8
pubmed: 25587948
Bioanalysis. 2014;6(14):1871-4
pubmed: 25158957
Pharmacopsychiatry. 2011 Sep;44(6):195-235
pubmed: 21969060
Rapid Commun Mass Spectrom. 2011 Oct 15;25(19):2951-8
pubmed: 21913274
J Occup Environ Med. 2014 Jul;56(7):e46-53
pubmed: 24988108
Anal Bioanal Chem. 2016 May;408(13):3503-9
pubmed: 26968564
J Chromatogr B Analyt Technol Biomed Life Sci. 2020 Jan 1;1136:121933
pubmed: 31846858
J Occup Environ Med. 2017 Nov;59(11):1114-1126
pubmed: 28930799
Anal Bioanal Chem. 2015 Jan;407(2):621-7
pubmed: 25374126
Br J Clin Pharmacol. 1983 Jul;16(1):17-25
pubmed: 6349668
Indian J Otolaryngol Head Neck Surg. 2011 Jul;63(3):255-9
pubmed: 22754805
J Pharm Biomed Anal. 2018 Feb 5;149:106-113
pubmed: 29112898
Clin Chem Lab Med. 2011 Jul;49(7):1091-103
pubmed: 21504375
Sleep Med Rev. 2010 Aug;14(4):259-67
pubmed: 20171127
Drug Test Anal. 2014 May;6(5):399-414
pubmed: 24692095
Anticancer Drugs. 2001 Nov;12(10):859-64
pubmed: 11707655
J Agric Food Chem. 2014 Jun 18;62(24):5485-91
pubmed: 24882375
Clin Chem. 2016 Apr;62(4):639-46
pubmed: 26888893
J Pharm Biomed Anal. 2016 Jan 25;118:70-80
pubmed: 26517851
Bioanalysis. 2016 Jun;8(11):1173-82
pubmed: 27212021
J Pharm Biomed Anal. 2016 May 10;123:132-40
pubmed: 26896632
J Pharm Biomed Anal. 2018 May 30;154:263-277
pubmed: 29579633
J Chromatogr A. 2018 Dec 7;1579:31-40
pubmed: 30430987
Bioanalysis. 2013 Aug;5(16):2023-41
pubmed: 23937137
J Hosp Med. 2013 Jan;8(1):1-6
pubmed: 23165956