Chimerism Monitoring Techniques after Hematopoietic Stem Cell Transplantation: An Overview of the Last 15 Years of Innovations.
NGS techniques
STR-PCR
chimerism analysis
dPCR
hematopoietic stem cell transplantation
qPCR
Journal
Diagnostics (Basel, Switzerland)
ISSN: 2075-4418
Titre abrégé: Diagnostics (Basel)
Pays: Switzerland
ID NLM: 101658402
Informations de publication
Date de publication:
30 Mar 2021
30 Mar 2021
Historique:
received:
24
02
2021
revised:
22
03
2021
accepted:
29
03
2021
entrez:
3
4
2021
pubmed:
4
4
2021
medline:
4
4
2021
Statut:
epublish
Résumé
Chimerism analysis is a well-established method for monitoring the state of hematopoietic stem cell transplantation (HSCT) over time by analyzing peripheral blood or bone marrow samples of the recipient in several malignant and non-malignant hematologic diseases. From a clinical point of view, a continuous monitoring is fundamental for an effective early therapeutic intervention. This paper provides a comparative overview of the main molecular biology techniques which can be used to study chimerism after bone marrow transplantation, focusing on their advantages and disadvantages. According to the examined literature, short tandem repeats (STR) analysis through simple PCR coupled with capillary electrophoresis (STR-PCR) is the most powerful method which guarantees a high power of differentiation between different individuals. However, other methods such as real-time quantitative PCR (qPCR), digital PCR (dPCR), and next-generation sequencing (NGS) technology were developed to overcome the technical limits of STR-PCR. In particular, these other techniques guarantee a higher sensitivity, which allows for the detection of chimerism at an earlier stage, hence expanding the window for therapeutic intervention. After a comparative evaluation of the various techniques, it seems clear that STR-PCR still remains the gold standard option for chimerism study, even if it is likely that both dPCR and NGS could supplement or even replace the common methods of STR analysis.
Identifiants
pubmed: 33808342
pii: diagnostics11040621
doi: 10.3390/diagnostics11040621
pmc: PMC8065704
pii:
doi:
Types de publication
Journal Article
Review
Langues
eng
Références
Hum Reprod. 2001 Jan;16(1):56-58
pubmed: 11139536
Am J Pharmacogenomics. 2004;4(3):177-87
pubmed: 15174899
Int J Lab Hematol. 2019 Oct;41(5):615-621
pubmed: 31225701
Clin Chem Lab Med. 2019 Apr 24;57(5):641-647
pubmed: 30457973
Int J Mol Sci. 2016 Sep 09;17(9):
pubmed: 27618030
Leukemia. 2012 Aug;26(8):1821-8
pubmed: 22395360
PLoS One. 2019 Feb 22;14(2):e0212708
pubmed: 30794643
Int J Lab Hematol. 2011 Feb;33(1):57-66
pubmed: 20681999
Rev Bras Hematol Hemoter. 2014 Sep-Oct;36(5):369-72
pubmed: 25305171
Acta Haematol. 2004;112(1-2):16-23
pubmed: 15179000
HLA. 2019 Dec;94 Suppl 2:16-20
pubmed: 31577854
Blood. 2004 Oct 15;104(8):2254-62
pubmed: 15226174
PLoS Med. 2009 Jul 21;6(7):e1000097
pubmed: 19621072
Bone Marrow Transplant. 2005 Jan;35(2):107-19
pubmed: 15502849
Mol Med Rep. 2016 Oct;14(4):2967-74
pubmed: 27499173
Exp Hematol. 2015 Jun;43(6):462-8.e1
pubmed: 25795523
Neoplasma. 2007;54(5):424-30
pubmed: 17688372
Appl Immunohistochem Mol Morphol. 2014 Jul;22(6):416-20
pubmed: 24569786
Int J Hematol. 2014 Mar;99(3):323-8
pubmed: 24481939
Transplant Cell Ther. 2021 Jan;27(1):89.e1-89.e10
pubmed: 32980546
Exp Hematol. 2010 Dec;38(12):1261-71
pubmed: 20851159
Hum Immunol. 2015 Mar;76(2-3):155-60
pubmed: 25636574
Isr Med Assoc J. 2007 Mar;9(3):159-62
pubmed: 17402326
Bone Marrow Transplant. 2004 Jul;34(1):1-12
pubmed: 15156163
Biomed Rep. 2014 Nov;2(6):804-808
pubmed: 25279149
Korean J Lab Med. 2009 Aug;29(4):277-81
pubmed: 19726887
Chimerism. 2014;5(2):24-39
pubmed: 24762743
J Egypt Natl Canc Inst. 2005 Jun;17(2):103-13
pubmed: 16508681
Blood Rev. 2020 Nov;44:100673
pubmed: 32173088
J Clin Med. 2019 Nov 28;8(12):
pubmed: 31795155
Clin Chim Acta. 2021 Jan;512:40-48
pubmed: 33227269
Hum Immunol. 2001 Jun;62(6):651-7
pubmed: 11390041
Int J Mol Sci. 2019 Sep 10;20(18):
pubmed: 31509957
Clin Chim Acta. 2017 May;468:51-59
pubmed: 28192074
Genes (Basel). 2020 Aug 25;11(9):
pubmed: 32854376
Bone Marrow Transplant. 2020 Jan;55(1):126-136
pubmed: 31455899
Biol Blood Marrow Transplant. 2005 Jul;11(7):558-66
pubmed: 15983556
Leukemia. 2005 Mar;19(3):336-43
pubmed: 15674363
Singapore Med J. 2007 Apr;48(4):333-7
pubmed: 17384881
Clin Chem Lab Med. 2013 Feb;51(2):363-9
pubmed: 23093278
Leukemia. 2011 Sep;25(9):1467-70
pubmed: 21681189
J Mol Diagn. 2019 May;21(3):483-490
pubmed: 30797064
Transfus Med Hemother. 2015 Jan;42(1):38-45
pubmed: 25960714
Am J Hematol. 2006 Oct;81(10):735-46
pubmed: 16838323
J Clin Med. 2020 Nov 25;9(12):
pubmed: 33255857
Clin Chem Lab Med. 2017 Jul 26;55(8):1115-1121
pubmed: 28099121
Eur J Haematol. 2006 Mar;76(3):237-44
pubmed: 16451397
Bone Marrow Transplant. 2020 Jul;55(7):1229-1239
pubmed: 32024991
Br J Haematol. 2015 Jan;168(1):26-37
pubmed: 25145701
HLA. 2018 Oct;92(4):215-223
pubmed: 30129247
Int J Legal Med. 2017 Jan;131(1):67-72
pubmed: 27448113
Br J Haematol. 2009 Mar;144(6):933-45
pubmed: 19183198
Korean J Hematol. 2011 Mar;46(1):18-23
pubmed: 21461299
Bone Marrow Transplant. 2005 Jun;35(11):1083-8
pubmed: 15821766
Blood. 2002 Jun 15;99(12):4618-25
pubmed: 12036896
Clin Chim Acta. 2011 Mar 18;412(7-8):625-30
pubmed: 21185273
J Mol Diagn. 2009 Jan;11(1):66-74
pubmed: 19056844
Expert Rev Mol Diagn. 2009 Nov;9(8):817-32
pubmed: 19895227
Biol Blood Marrow Transplant. 2014 Dec;20(12):1918-25
pubmed: 25087899