CUTie2: The Attack of the Cyclic Nucleotide Sensor Clones.
CUTie
FRET
Sirah
biosensor
coarse-grained
cyclic nucleotide
molecular dynamics
signaling
Journal
Frontiers in molecular biosciences
ISSN: 2296-889X
Titre abrégé: Front Mol Biosci
Pays: Switzerland
ID NLM: 101653173
Informations de publication
Date de publication:
2021
2021
Historique:
received:
15
11
2020
accepted:
20
01
2021
entrez:
29
3
2021
pubmed:
30
3
2021
medline:
30
3
2021
Statut:
epublish
Résumé
The detection of small molecules in living cells using genetically encoded FRET sensors has revolutionized our understanding of signaling pathways at the sub-cellular level. However, engineering fluorescent proteins and specific binding domains to create new sensors remains challenging because of the difficulties associated with the large size of the polypeptides involved, and their intrinsically huge conformational variability. Indeed, FRET sensors' design still relies on vague structural notions, and trial and error combinations of linkers and protein modules. We recently designed a FRET sensor for the second messenger cAMP named CUTie (
Identifiants
pubmed: 33778003
doi: 10.3389/fmolb.2021.629773
pii: 629773
pmc: PMC7991088
doi:
Types de publication
Journal Article
Langues
eng
Pagination
629773Informations de copyright
Copyright © 2021 Klein, Sardi, Machado, Ortega, Comini and Pantano.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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