Mitochondrial movement during its association with chloroplasts in Arabidopsis thaliana.
Actin Cytoskeleton
/ physiology
Actins
/ metabolism
Arabidopsis
/ genetics
Chloroplast Proton-Translocating ATPases
/ genetics
Chloroplasts
/ metabolism
Luminescent Proteins
/ genetics
Mesophyll Cells
/ metabolism
Microscopy, Confocal
Microscopy, Video
Mitochondria
/ metabolism
Movement
Plants, Genetically Modified
/ genetics
Recombinant Fusion Proteins
/ metabolism
Time Factors
Time-Lapse Imaging
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
05 03 2021
05 03 2021
Historique:
received:
11
02
2020
accepted:
11
02
2021
entrez:
6
3
2021
pubmed:
7
3
2021
medline:
11
8
2021
Statut:
epublish
Résumé
Plant mitochondria move dynamically inside cells and this movement is classified into two types: directional movement, in which mitochondria travel long distances, and wiggling, in which mitochondria travel short distances. However, the underlying mechanisms and roles of both types of mitochondrial movement, especially wiggling, remain to be determined. Here, we used confocal laser-scanning microscopy to quantitatively characterize mitochondrial movement (rate and trajectory) in Arabidopsis thaliana mesophyll cells. Directional movement leading to long-distance migration occurred at high speed with a low angle-change rate, whereas wiggling leading to short-distance migration occurred at low speed with a high angle-change rate. The mean square displacement (MSD) analysis could separate these two movements. Directional movement was dependent on filamentous actin (F-actin), whereas mitochondrial wiggling was not, but slightly influenced by F-actin. In mesophyll cells, mitochondria could migrate by wiggling, and most of these mitochondria associated with chloroplasts. Thus, mitochondria migrate via F-actin-independent wiggling under the influence of F-actin during their association with chloroplasts in Arabidopsis.
Identifiants
pubmed: 33674706
doi: 10.1038/s42003-021-01833-8
pii: 10.1038/s42003-021-01833-8
pmc: PMC7935954
doi:
Substances chimiques
Actins
0
Luminescent Proteins
0
Recombinant Fusion Proteins
0
Chloroplast Proton-Translocating ATPases
EC 3.6.3.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Video-Audio Media
Langues
eng
Sous-ensembles de citation
IM
Pagination
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