Detection of Tumor-Associated Membrane Receptors on Extracellular Vesicles from Non-Small Cell Lung Cancer Patients via Immuno-PCR.
EGFR
affibody
biomarkers
immuno-PCR
liquid biopsies
longitudinal treatment monitoring
non-small-cell lung cancer
protein–DNA conjugation
small extracellular vesicles
tyrosine kinase inhibitors
Journal
Cancers
ISSN: 2072-6694
Titre abrégé: Cancers (Basel)
Pays: Switzerland
ID NLM: 101526829
Informations de publication
Date de publication:
22 Feb 2021
22 Feb 2021
Historique:
received:
30
12
2020
revised:
11
02
2021
accepted:
18
02
2021
entrez:
6
3
2021
pubmed:
7
3
2021
medline:
7
3
2021
Statut:
epublish
Résumé
Precision cancer medicine for non-small-cell lung cancer (NSCLC) has increased patient survival. Nevertheless, targeted agents towards tumor-associated membrane receptors only result in partial remission for a limited time, calling for approaches which allow longitudinal treatment monitoring. Rebiopsy of tumors in the lung is challenging, and metastatic lesions may have heterogeneous signaling. One way ahead is to use liquid biopsies such as circulating tumor DNA or small extracellular vesicles (sEVs) secreted by the tumor into blood or other body fluids. Herein, an immuno-PCR-based detection of the tumor-associated membrane receptors EGFR, HER2, and IGF-1R on CD9-positive sEVs from NSCLC cells and pleural effusion fluid (PE) of NSCLC patients is developed utilizing DNA conjugates of antibody mimetics and affibodies, as detection agents. Results on sEVs purified from culture media of NSCLC cells treated with anti-EGFR siRNA, showed that the reduction of EGFR expression can be detected via immuno-PCR. Protein profiling of sEVs from NSCLC patient PE samples revealed the capacity to monitor EGFR, HER2, and IGF-1R with the immuno-PCR method. We detected a significantly higher EGFR level in sEVs derived from a PE sample of a patient with an
Identifiants
pubmed: 33671772
pii: cancers13040922
doi: 10.3390/cancers13040922
pmc: PMC7926549
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Vetenskapsrådet
ID : 2016-05207
Organisme : Familjen Erling-Perssons Stiftelse
ID : 2019.12.17
Organisme : Cancerföreningen i Stockholm
ID : 191293
Organisme : Cancerföreningen i Stockholm
ID : 161283
Organisme : Cancerföreningen i Stockholm
ID : 171123
Organisme : Cancerföreningen i Stockholm
ID : 151122
Organisme : Cancerfonden
ID : CAN 2015/401
Organisme : Cancerfonden
ID : CAN 2018/597
Organisme : Stockholms Läns Landsting
ID : 20160287
Organisme : Stockholms Läns Landsting
ID : 20180404
Organisme : Karolinska FOUU
ID : 75032
Références
ACS Sens. 2019 May 24;4(5):1399-1408
pubmed: 31020844
Cancer Cell. 2017 Feb 13;31(2):172-179
pubmed: 28196593
Sci Adv. 2020 Mar 11;6(11):eaaz6162
pubmed: 32195353
Lung Cancer. 2020 Sep;147:123-129
pubmed: 32693293
Lung Cancer (Auckl). 2017 Aug 18;8:109-125
pubmed: 28860885
Adv Biosyst. 2020 Dec;4(12):e1900305
pubmed: 32394646
Mol Cancer. 2018 Jan 27;17(1):15
pubmed: 29374476
Mol Oncol. 2016 Dec;10(10):1595-1602
pubmed: 27856179
Clin Cancer Res. 2018 Jun 15;24(12):2944-2950
pubmed: 29535126
PLoS One. 2013 May 10;8(5):e62791
pubmed: 23675426
J Thorac Dis. 2018 Apr;10(Suppl 7):S882-S897
pubmed: 29780635
Chem Rev. 2018 Feb 28;118(4):1917-1950
pubmed: 29384376
Ann Transl Med. 2018 Apr;6(8):141
pubmed: 29862230
Ann Oncol. 2018 Mar 1;29(3):700-706
pubmed: 29216356
J Mol Biol. 2008 Mar 7;376(5):1388-402
pubmed: 18207161
Cancers (Basel). 2018 Feb 28;10(3):
pubmed: 29495603
Biosens Bioelectron. 2020 Apr 15;154:112056
pubmed: 32093894
Adv Drug Deliv Rev. 2018 Feb 1;125:102-121
pubmed: 29355669
Expert Rev Mol Diagn. 2018 Dec;18(12):1029-1040
pubmed: 30406709
Curr Oncol. 2018 Jun;25(Suppl 1):S68-S76
pubmed: 29910649
J Thorac Oncol. 2018 Sep;13(9):1248-1268
pubmed: 29885479
N Engl J Med. 2018 Jan 11;378(2):113-125
pubmed: 29151359
J Extracell Vesicles. 2014 Dec 22;3:26913
pubmed: 25536934
Mol Biol Rep. 2020 Oct;47(10):8179-8187
pubmed: 33029702
Exp Mol Med. 2017 Mar 24;49(3):e306
pubmed: 28336959
Liver Int. 2020 Dec;40(12):3103-3116
pubmed: 32614460
Theranostics. 2019 Jul 28;9(19):5532-5541
pubmed: 31534501
N Engl J Med. 2020 Jan 2;382(1):41-50
pubmed: 31751012
Cancer Res. 2006 Apr 15;66(8):4339-48
pubmed: 16618759
Expert Rev Anticancer Ther. 2017 Sep;17(9):779-786
pubmed: 28701107
Cancers (Basel). 2020 May 02;12(5):
pubmed: 32370304
Transl Lung Cancer Res. 2019 Dec;8(6):1051-1060
pubmed: 32010582
Bioconjug Chem. 2019 Nov 20;30(11):2790-2798
pubmed: 31609586
Nat Commun. 2020 Sep 14;11(1):4607
pubmed: 32929081
J Extracell Vesicles. 2018 Nov 23;7(1):1535750
pubmed: 30637094
Adv Clin Chem. 2018;83:73-119
pubmed: 29304904
Lung Cancer. 2018 Oct;124:45-52
pubmed: 30268479
Br J Cancer. 2008 Sep 16;99(6):911-22
pubmed: 19238632
Biotechnol Appl Biochem. 2010 Feb 25;55(2):99-109
pubmed: 20088825
Sci Rep. 2020 Jan 9;10(1):92
pubmed: 31919420
Protein Eng Des Sel. 2004 May;17(5):455-62
pubmed: 15208403
Eur J Nucl Med Mol Imaging. 2010 Mar;37(3):613-22
pubmed: 19838701
Mol Cancer. 2018 Feb 19;17(1):38
pubmed: 29455650
J Nucl Med. 2012 Jan;53(1):90-7
pubmed: 22173843
Front Neurol. 2015 Aug 19;6:179
pubmed: 26347708