Evaluation of B Cell Proliferation
B cell
EdU
Flow cytometry
Germinal center
Proliferation
Subepithelial dome
Journal
Bio-protocol
ISSN: 2331-8325
Titre abrégé: Bio Protoc
Pays: United States
ID NLM: 101635102
Informations de publication
Date de publication:
05 May 2020
05 May 2020
Historique:
received:
30
11
2019
revised:
22
03
2020
accepted:
23
03
2020
entrez:
4
3
2021
pubmed:
5
3
2021
medline:
5
3
2021
Statut:
epublish
Résumé
Generation of antibodies is crucial for establishing enduring protection from invading pathogens, as well as for maintaining homeostasis with commensal bacteria at mucosal surfaces. Chronic exposure to microbiota- and dietary- derived antigens results in continuous production of antibody producing cells within the Peyer's patch germinal center structures. Recently, we have shown that B cells responding to gut-derived antigens colonize the subepithelial dome (SED) in Peyer's patches and rapidly proliferate independently of their relative BCR affinity. To evaluate B cell proliferation within different niches in Peyer's patches, we applied
Identifiants
pubmed: 33659568
doi: 10.21769/BioProtoc.3602
pii: e3602
pmc: PMC7842390
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e3602Informations de copyright
Copyright © 2020 The Authors; exclusive licensee Bio-protocol LLC.
Déclaration de conflit d'intérêts
Competing interestsThe author declare no competing interests.
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