PEG-mediated, Stable, Nuclear and Chloroplast Transformation of

Chloramphenicol selection Chloroplast transformation Cyanidioschizon merolae Mutant selection Nuclear transformation PEG mediated vector delivery

Journal

Bio-protocol
ISSN: 2331-8325
Titre abrégé: Bio Protoc
Pays: United States
ID NLM: 101635102

Informations de publication

Date de publication:
05 Sep 2019
Historique:
received: 22 03 2019
revised: 22 08 2019
accepted: 22 08 2019
entrez: 3 3 2021
pubmed: 5 9 2019
medline: 5 9 2019
Statut: epublish

Résumé

The ability to achieve nuclear or chloroplast transformation in plants has been a long standing goal, especially in microalgae research. Over past years there has been only little success, but transient and stable nuclear transformation has been achieved in multiple species. Our newly developed method allows for relatively simple transformation of

Identifiants

pubmed: 33654854
doi: 10.21769/BioProtoc.3355
pii: e3355
pmc: PMC7854100
doi:

Types de publication

Journal Article

Langues

eng

Pagination

e3355

Informations de copyright

Copyright © 2019 The Authors; exclusive licensee Bio-protocol LLC.

Déclaration de conflit d'intérêts

Competing interestsNo competing interests declared.

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Auteurs

Maksymilian Zienkiewicz (M)

Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland.

Tomasz Krupnik (T)

Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland.

Anna Drożak (A)

Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland.

Kinga Kania (K)

Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland.

Classifications MeSH