Gastric Enzyme Supplementation Inhibits Food Allergy in a BALB/c Mouse Model.
Allergens
/ immunology
Animals
Antibodies
/ blood
B-Lymphocyte Subsets
/ immunology
Cytokines
/ blood
Dietary Supplements
Digestion
/ immunology
Disease Models, Animal
Food Hypersensitivity
/ immunology
Gastrointestinal Agents
/ administration & dosage
Immune Tolerance
/ drug effects
Lymphocyte Count
Mice
Mice, Inbred BALB C
Ovalbumin
/ immunology
Stomach
/ immunology
T-Lymphocytes, Regulatory
/ immunology
allergy prevention
food allergy
gastric acid suppression
gastric enzymes
systemic allergic response
Journal
Nutrients
ISSN: 2072-6643
Titre abrégé: Nutrients
Pays: Switzerland
ID NLM: 101521595
Informations de publication
Date de publication:
26 Feb 2021
26 Feb 2021
Historique:
received:
29
12
2020
revised:
08
02
2021
accepted:
22
02
2021
entrez:
3
3
2021
pubmed:
4
3
2021
medline:
11
5
2021
Statut:
epublish
Résumé
Impaired gastric digestion due to suppressed gastric acidity enhances the risk for food allergy development. In the current study, we aimed to evaluate the impact of a supported gastric digestion via application of a pharmaceutical gastric enzyme solution (GES) on food allergy development and allergic reactions in a BALB/c mouse model. The ability of the GES to restore hypoacidic conditions was tested in mice treated with gastric acid suppression medication. To evaluate the impact on allergic symptoms, mice were orally sensitized with ovalbumin (OVA) under gastric acid suppression and subjected to oral challenges with or without GES. The immune response was evaluated by measurement of antibody titers, cytokine levels, mucosal allergy effector cell influx and regulatory T-cell counts. Clinical response was objectified by core body temperature measurements after oral OVA challenge. Supplementation of GES transiently restored physiological pH levels in the stomach after pharmaceutical gastric acid suppression. During oral sensitization, supplementation of gastric enzymes significantly reduced systemic IgE, IgG1 and IgG2a levels and allergic symptoms. In food allergic mice, clinical symptoms were reduced by co-administration of the gastric enzyme solution. Support of gastric digestion efficiently prevents food allergy induction and alleviates clinical symptoms in our food allergy model.
Identifiants
pubmed: 33652629
pii: nu13030738
doi: 10.3390/nu13030738
pmc: PMC7996948
pii:
doi:
Substances chimiques
Allergens
0
Antibodies
0
Cytokines
0
Gastrointestinal Agents
0
Ovalbumin
9006-59-1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Nordmark Arzneimittel GmbH & Co. KG, Uetersen, Germany
ID : no grant number available
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