The effect of maternal HMB supplementation on bone mechanical and geometrical properties, as well as histomorphometry and immunolocalization of VEGF, TIMP2, MMP13, BMP2 in the bone and cartilage tissue of the humerus of their newborn piglets.
Animal Feed
/ analysis
Animal Nutritional Physiological Phenomena
Animals
Animals, Newborn
/ embryology
Bone Morphogenetic Protein 2
/ metabolism
Bone and Bones
/ drug effects
Cartilage
Diet
/ veterinary
Dietary Supplements
Female
Humerus
/ drug effects
Male
Maternal Exposure
Matrix Metalloproteinase 13
/ metabolism
Poland
Pregnancy
Swine
/ embryology
Tissue Inhibitor of Metalloproteinase-2
/ metabolism
Valerates
/ metabolism
Vascular Endothelial Growth Factor A
/ metabolism
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2021
2021
Historique:
received:
28
09
2020
accepted:
06
02
2021
entrez:
24
2
2021
pubmed:
25
2
2021
medline:
9
7
2021
Statut:
epublish
Résumé
The presented experiment focuses on assessing the impact of HMB (hydroxy-β-methobutyrate) supplementation of mothers during pregnancy on the development of the skeletal system of their offspring. For this purpose, an experiment was carried out on 12 clinically healthy sows of the Great White Poland breed, which were divided randomly into two groups the control and the HMB group. All animals were kept under standard conditions and received the same feed for pregnant females. In contrast, females from the HMB group between 70 and 90 days were supplemented with 3-hydroxy-3-methylbutyle in the amount of 0.2g/kg b.w/day. Immediately after birth, the piglets were also divided into groups based on: sex, and presence or lack HMB supplementation, and subsequently were euthanized and humerus bones from all piglets were collected. Mother's HMB supplementation during pregnancy affected the multiple index of their offspring. The higher humerus mass and length was observed with the greater effect in males. Maternal supplementation also influenced on the geometrical and mechanical properties of the humerus as in the case of mass, this effect was higher in males. Also, the collagen structure of the compacted and trabecular bone changed under the HMB addition. Maternal supplementation also affected the expression of selected proteins in growth cartilage and trabecular bone. The obtained results show that the administration to the mother during pregnancy by the HMB significantly affects the development of the humerus in many ways. The obtained results also confirm the utility of such experiments in understanding of the importance of the pregnancy diet as an develop and adaptable factor of offspring organisms and are the base for further research in that area as well as in the protein markers expression area.
Identifiants
pubmed: 33626093
doi: 10.1371/journal.pone.0240642
pii: PONE-D-20-30549
pmc: PMC7904207
doi:
Substances chimiques
Bone Morphogenetic Protein 2
0
Valerates
0
Vascular Endothelial Growth Factor A
0
Tissue Inhibitor of Metalloproteinase-2
127497-59-0
beta-hydroxyisovaleric acid
3F752311CD
Matrix Metalloproteinase 13
EC 3.4.24.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0240642Déclaration de conflit d'intérêts
NO authors have competing interests.
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