Automated 3D Microphysiometry Facilitates High-Content and Highly Reproducible Oxygen Measurements within 3D Cell Culture Models.

Cell Culture Techniques Humans Oxygen Printing, Three-Dimensional
3D cell culture biosensors gelatin methacryloyl (GelMA) hydrogels label-free metabolic monitoring oxygenation

Journal

ACS sensors
ISSN: 2379-3694
Titre abrégé: ACS Sens
Pays: United States
ID NLM: 101669031

Informations de publication

Date de publication:
26 03 2021
Historique:
pubmed: 24 2 2021
medline: 15 5 2021
entrez: 23 2 2021
Statut: ppublish

Résumé

Microphysiometry is a powerful technique to study metabolic parameters and detect changes to external stimuli. However, applying this technique for automated label-free and real-time measurements within cell-laden three-dimensional (3D) cell culture constructs remains a challenge. Herein, we present an entirely automated microphysiometry setup that combines needle-type microsensors with motorized sample and sensor positioning systems inside a standard tissue-culture incubator. The setup records dissolved oxygen as a metabolic parameter along the

Identifiants

DOI: 10.1021/acssensors.0c02551 PMID: 33621068
pubmed: 33621068
doi: 10.1021/acssensors.0c02551
doi:

Substances chimiques

Oxygen S88TT14065

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1248-1260

Auteurs

Sebastian Eggert (S)

Centre in Regenerative Medicine, Queensland University of Technology, Brisbane, 4000 QLD, Australia.
School of Mechanical, Medical and Process Engineering, Science and Engineering Faculty, Queensland University of Technology, Brisbane, 4000 QLD, Australia.
Chair of Medical Materials and Implants, Department of Mechanical Engineering and Munich School of BioEngineering, Technical University of Munich, Garching 85748, Germany.
ORCID: 0000-0001-9057-9922

Martin S Gutbrod (MS)

PreSens Precision Sensing GmbH, Am Biopark 11, 93053 Regensburg, Germany.

Gregor Liebsch (G)

PreSens Precision Sensing GmbH, Am Biopark 11, 93053 Regensburg, Germany.

Robert Meier (R)

PreSens Precision Sensing GmbH, Am Biopark 11, 93053 Regensburg, Germany.

Christoph Meinert (C)

Centre in Regenerative Medicine, Queensland University of Technology, Brisbane, 4000 QLD, Australia.
School of Mechanical, Medical and Process Engineering, Science and Engineering Faculty, Queensland University of Technology, Brisbane, 4000 QLD, Australia.
ORCID: 0000-0002-7036-4067

Dietmar W Hutmacher (DW)

Centre in Regenerative Medicine, Queensland University of Technology, Brisbane, 4000 QLD, Australia.
School of Mechanical, Medical and Process Engineering, Science and Engineering Faculty, Queensland University of Technology, Brisbane, 4000 QLD, Australia.
ARC ITTC in Additive Biomanufacturing, Queensland University of Technology, Brisbane, 4000 QLD, Australia.
ORCID: 0000-0001-5678-2134

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Classifications MeSH

Recherchemedicale.com › Techniques d'investigation › Techniques in vitro › Techniques de culture › Techniques de culture cellulaire › Automated 3D Microphysiometry Facilitates...
Recherchemedicale.com › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Automated 3D Microphysiometry Facilitates...
Recherchemedicale.com › Produits chimiques inorganiques › Gaz › Oxygène › Automated 3D Microphysiometry Facilitates...
Recherchemedicale.com › Sciences de l'information › Affichage de données › Infographie › Conception assistée par ordinateur › Impression tridimensionnelle › Automated 3D Microphysiometry Facilitates...