Whole-genome analyses of extended-spectrum or AmpC β-lactamase-producing Escherichia coli isolates from companion dogs in Japan.


Journal

PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081

Informations de publication

Date de publication:
2021
Historique:
received: 13 11 2020
accepted: 19 01 2021
entrez: 5 2 2021
pubmed: 6 2 2021
medline: 31 7 2021
Statut: epublish

Résumé

The emergence and global spread of extended-spectrum or AmpC β-lactamase (ESBL/AmpC)-producing Enterobacteriaceae in companion animals have led to the hypothesis that companion animals might be reservoirs for cross-species transmission because of their close contact with humans. However, current knowledge in this field is limited; therefore, the role of companion animals in cross-species transmission remains to be elucidated. Herein, we studied ESBL/AmpC-producing Enterobacteriaceae, Escherichia coli in particular, isolated from extraintestinal sites and feces of companion dogs. Whole-genome sequencing analysis revealed that (i) extraintestinal E. coli isolates were most closely related to those isolated from feces from the same dog, (ii) chromosomal sequences in the ST131/C1-M27 clade isolated from companion dogs were highly similar to those in the ST131/C1-M27 clade of human origin, (iii) certain plasmids, such as IncFII/pMLST F1:A2:B20/blaCTX-M-27, IncI1/pMLST16/blaCTX-M-15, or IncI1/blaCMY-2 from dog-derived E. coli isolates, shared high homology with those from several human-derived Enterobacteriaceae, (iv) chromosomal blaCTX-M-14 was identified in the ST38 isolate from a companion dog, and (v) eight out of 14 tested ESBL/AmpC-producing E. coli isolates (i.e., ST131, ST68, ST405, and ST998) belonged to the human extraintestinal pathogenic E. coli (ExPEC) group. All of the bla-coding plasmids that were sequenced genome-wide were capable of horizontal transfer. These results suggest that companion dogs can spread ESBL/AmpC-producing ExPEC via their feces. Furthermore, at least some ESBL/AmpC-producing ExPECs and bla-coding plasmids can be transmitted between humans and companion dogs. Thus, companion dogs can act as an important reservoir for ESBL/AmpC-producing E. coli in the community.

Identifiants

pubmed: 33544781
doi: 10.1371/journal.pone.0246482
pii: PONE-D-20-35693
pmc: PMC7864471
doi:

Substances chimiques

Anti-Bacterial Agents 0
Bacterial Proteins 0
AmpC beta-lactamases EC 3.5.2.6
beta-Lactamases EC 3.5.2.6

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e0246482

Déclaration de conflit d'intérêts

The authors have declared that no competing interests exist.

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Auteurs

Mayo Yasugi (M)

Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan.
Asian Health Science Research Institiute, Osaka Prefecture University, Izumisano, Osaka, Japan.

Shingo Hatoya (S)

Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan.

Daisuke Motooka (D)

Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan.

Yuki Matsumoto (Y)

Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan.

Shunsuke Shimamura (S)

Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan.

Hiroyuki Tani (H)

Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan.

Masaru Furuya (M)

Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan.

Keiichiro Mie (K)

Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan.

Masami Miyake (M)

Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan.

Shota Nakamura (S)

Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan.

Terumasa Shimada (T)

Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan.
Asian Health Science Research Institiute, Osaka Prefecture University, Izumisano, Osaka, Japan.

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