Noninvasive imaging and quantification of bile salt hydrolase activity: From bacteria to humans.
Journal
Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440
Informations de publication
Date de publication:
02 2021
02 2021
Historique:
received:
25
10
2019
accepted:
16
12
2020
entrez:
4
2
2021
pubmed:
5
2
2021
medline:
19
4
2022
Statut:
epublish
Résumé
The microbiome-produced enzyme bile salt hydrolase (BSH) plays a central role in human health, but its function remains unclear due to the lack of suitable methods for measuring its activity. Here, we have developed a novel optical tool based on ultrasensitive bioluminescent imaging and demonstrated that this assay can be used for quick and cost-effective quantification of BSH activity across a broad range of biological settings including pure enzymes and bacteria, intact fecal slurries, and noninvasive imaging in live animals, as well as for the assessment of BSH activity in the entire gastrointestinal tract of mice and humans. Using this assay, we showed that certain types of prebiotics are capable of increasing BSH activity of the gut microbiota in vivo and successfully demonstrated potential application of this assay as a noninvasive diagnostic test to predict the clinical status of inflammatory bowel disease (IBD) patients.
Identifiants
pubmed: 33536224
pii: 7/6/eaaz9857
doi: 10.1126/sciadv.aaz9857
pmc: PMC7857686
pii:
doi:
Substances chimiques
Bile Acids and Salts
0
Prebiotics
0
Amidohydrolases
EC 3.5.-
choloylglycine hydrolase
EC 3.5.1.24
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
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