Benefits of Iterative Searches of Large Databases to Interpret Large Human Gut Metaproteomic Data Sets.
X!Tandem
database search
gut microbiome
metaproteomics
Journal
Journal of proteome research
ISSN: 1535-3907
Titre abrégé: J Proteome Res
Pays: United States
ID NLM: 101128775
Informations de publication
Date de publication:
05 03 2021
05 03 2021
Historique:
pubmed:
3
2
2021
medline:
22
6
2021
entrez:
2
2
2021
Statut:
ppublish
Résumé
The gut microbiota are increasingly considered as a main partner of human health. Metaproteomics enables us to move from the functional potential revealed by metagenomics to the functions actually operating in the microbiome. However, metaproteome deciphering remains challenging. In particular, confident interpretation of a myriad of MS/MS spectra can only be pursued with smart database searches. Here, we compare the interpretation of MS/MS data sets from 48 individual human gut microbiomes using three interrogation strategies of the dedicated Integrated nonredundant Gene Catalog (IGC 9.9 million genes from 1267 individual fecal samples) together with the
Identifiants
pubmed: 33528260
doi: 10.1021/acs.jproteome.0c00669
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM