Role of circulating endothelial cells in assessing the severity of systemic sclerosis and predicting its clinical worsening.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
29 01 2021
Historique:
received: 29 01 2020
accepted: 09 12 2020
entrez: 30 1 2021
pubmed: 31 1 2021
medline: 16 11 2021
Statut: epublish

Résumé

Endothelial damage and fibro-proliferative vasculopathy of small vessels are pathological hallmarks of systemic sclerosis (SSc). The consequence is the detachment of resident elements that become circulating endothelial cells (CECs). The aim of our study was to evaluate the potential of CECs as biomarker in SSc. We enrolled 50 patients with limited cutaneous (lcSSc) and diffuse cutaneous (dcSSc) subset of SSc, who underwent clinical evaluation to establish the organ involvement. CECs were measured by flow-cytometry utilizing a polychromatic panel. An evident difference was observed in CEC counts comparing controls to SSc patients (median 10.5 vs. 152 cells/ml, p < 0.0001) and for the first time, between the two subsets of disease (median lcSSc 132 vs. dcSSc 716 CEC/ml, p < 0.0001). A significant correlation was established between CECs and some SSc clinical parameters, such as digital ulcers, skin and pulmonary involvement, presence of Scl-70 antibodies, nailfold videocapillaroscopy patterns and EUSTAR activity index. After 12 months, CECs correlated with clinical worsening of patients, showing that a number higher than 414 CEC/ml is a strong negative prognostic factor (RR 5.70). Our results indicate that CECs are a direct indicator of systemic vascular damage. Therefore, they can be used as a reliable marker of disease severity.

Identifiants

pubmed: 33514797
doi: 10.1038/s41598-020-80604-7
pii: 10.1038/s41598-020-80604-7
pmc: PMC7846576
doi:

Types de publication

Clinical Trial Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

2681

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Auteurs

Maria Luisa Di Martino (ML)

Department of Medical Sciences and Public Health and Unit of Internal Medicine, University of Cagliari, SS554-km 4,500, 09042, Monserrato, Cagliari, Italy. m.luisadimartino@gmail.com.

Alessandra Frau (A)

Department of Medical Sciences and Public Health and Unit of Internal Medicine, University of Cagliari, SS554-km 4,500, 09042, Monserrato, Cagliari, Italy.

Francesca Losa (F)

Department of Medical Sciences and Public Health and Unit of Internal Medicine, University of Cagliari, SS554-km 4,500, 09042, Monserrato, Cagliari, Italy.

Emma Muggianu (E)

Department of Medical Sciences and Public Health and Unit of Internal Medicine, University of Cagliari, SS554-km 4,500, 09042, Monserrato, Cagliari, Italy.

Mario Nicola Mura (MN)

Department of Medical Sciences and Public Health and Unit of Internal Medicine, University of Cagliari, SS554-km 4,500, 09042, Monserrato, Cagliari, Italy.

Gianluca Rotta (G)

Becton Dickinson Biosciences Italy, Milan, Italy.

Lorenza Scotti (L)

Department of Translational Medicine, University of Piemonte Orientale, Novara, Italy.

Francesco Marongiu (F)

Department of Medical Sciences and Public Health and Unit of Internal Medicine, University of Cagliari, SS554-km 4,500, 09042, Monserrato, Cagliari, Italy.

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Classifications MeSH