Three-Dimensional Imaging of Pulmonary Fibrotic Foci at the Alveolar Scale Using Tissue-Clearing Treatment with Staining Techniques of Extracellular Matrix.
Journal
International journal of biomedical imaging
ISSN: 1687-4188
Titre abrégé: Int J Biomed Imaging
Pays: United States
ID NLM: 101250756
Informations de publication
Date de publication:
2020
2020
Historique:
received:
13
04
2020
revised:
09
12
2020
accepted:
16
12
2020
entrez:
18
1
2021
pubmed:
19
1
2021
medline:
19
1
2021
Statut:
epublish
Résumé
Idiopathic pulmonary fibrosis is a progressive, chronic lung disease characterized by the accumulation of extracellular matrix proteins, including collagen and elastin. Imaging of extracellular matrix in fibrotic lungs is important for evaluating its pathological condition as well as the distribution of drugs to pulmonary focus sites and their therapeutic effects. In this study, we compared techniques of staining the extracellular matrix with optical tissue-clearing treatment for developing three-dimensional imaging methods for focus sites in pulmonary fibrosis. Mouse models of pulmonary fibrosis were prepared via the intrapulmonary administration of bleomycin. Fluorescent-labeled tomato lectin, collagen I antibody, and Col-F, which is a fluorescent probe for collagen and elastin, were used to compare the imaging of fibrotic foci in intact fibrotic lungs. These lung samples were cleared using the Clear
Identifiants
pubmed: 33456450
doi: 10.1155/2020/8815231
pmc: PMC7787752
doi:
Types de publication
Journal Article
Langues
eng
Pagination
8815231Informations de copyright
Copyright © 2020 Kohei Togami et al.
Déclaration de conflit d'intérêts
The authors declare that there is no conflict of interest regarding the publication of this paper.
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