Combinatorial expression of cell cycle regulators is more suitable for immortalization than oncogenic methods in dermal papilla cells.
Cell Biology
Stem Cell Research
Technical Aspects of Cell Biology
Journal
iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038
Informations de publication
Date de publication:
22 Jan 2021
22 Jan 2021
Historique:
received:
29
05
2020
revised:
05
11
2020
accepted:
08
12
2020
entrez:
13
1
2021
pubmed:
14
1
2021
medline:
14
1
2021
Statut:
epublish
Résumé
The immortalized cell is an essential research tool that uses robust growth properties for the functional investigation of gene products. Immortalized mammalian cells have mainly been established using three methods: expression of simian vacuolating virus 40 T antigen (the SV40 method); human papilloma virus-derived oncoprotein E6/E7 (the E6/E7 method); or combinatorial expression of R24C mutant cyclin-dependent kinase 4, cyclin D1, and telomerase reverse transcriptase (the K4DT method). However, it is unclear as to which method is optimal for an
Identifiants
pubmed: 33437932
doi: 10.1016/j.isci.2020.101929
pii: S2589-0042(20)31126-3
pmc: PMC7788094
doi:
Banques de données
figshare
['10.6084/m9.figshare.12197691.v2']
Types de publication
Journal Article
Langues
eng
Pagination
101929Informations de copyright
© 2020 The Author(s).
Déclaration de conflit d'intérêts
The authors declare no conflict of interests.
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