The usefulness of the decellularized matrix from three-dimensional regenerative cartilage as a scaffold material.
3D-culture
Decellularization
Extracellular matrix
Recellularization
Regenerative cartilage
Scaffold
Journal
Regenerative therapy
ISSN: 2352-3204
Titre abrégé: Regen Ther
Pays: Netherlands
ID NLM: 101709085
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
15
07
2020
revised:
30
10
2020
accepted:
18
11
2020
entrez:
11
1
2021
pubmed:
12
1
2021
medline:
12
1
2021
Statut:
epublish
Résumé
In cartilage tissue engineering, research on materials for three-dimensional (3D) scaffold has attracted attention. Decellularized matrix can be one of the candidates for the scaffold material. In this study, decellularization of regenerated cartilage was carried out and its effectiveness as a scaffold material was examined. Three-dimensionally-cultured cartilage constructs in the differentiation medium containing IGF-1 produced more cartilage matrix than those in the proliferation medium. Detergent-enzymatic method (DEM) could decellularize 3D-cultured cartilage constructs only by 1 cycle without breaking down the structure of the constructs. In vitro, newly-seeded chondrocytes were infiltrated and engrafted into decellularized constructs in the proliferation medium, and newly formed fibers were observed around the surface where newly-seeded cells were attached. Recellularized constructs could mature similarly as those without decellularization in vivo. The decellularized 3D-cultured matrix from regenerative cartilage is expected to be used as a scaffold material in the future.
Identifiants
pubmed: 33426234
doi: 10.1016/j.reth.2020.11.005
pii: S2352-3204(20)30088-2
pmc: PMC7770429
doi:
Types de publication
Journal Article
Langues
eng
Pagination
312-322Informations de copyright
© 2020 The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V.
Déclaration de conflit d'intérêts
Atsuhiko Hikita and Yukiyo Asawa: Affiliation with an endowed chair from FUJISOFT INCORPORATED.
Références
Transpl Int. 2005 Jun;18(6):727-34
pubmed: 15910302
Science. 2010 Jul 30;329(5991):538-41
pubmed: 20576850
Clin Orthop Relat Res. 1984 Jun;(186):231-9
pubmed: 6723151
Nature. 1977 Jun 9;267(5611):531-2
pubmed: 559947
Adv Healthc Mater. 2015 Jan 7;4(1):29-39
pubmed: 25044502
Ann N Y Acad Sci. 2016 Nov;1383(1):139-159
pubmed: 27870078
Immunol Res. 2015 Dec;63(1-3):181-6
pubmed: 26481914
Biomaterials. 2006 Jul;27(19):3675-83
pubmed: 16519932
Tissue Eng Part A. 2016 Apr;22(7-8):665-79
pubmed: 27001140
Cell Transplant. 2012;21(7):1431-42
pubmed: 22546666
Curr Opin Otolaryngol Head Neck Surg. 2019 Aug;27(4):261-266
pubmed: 31082936
Regen Ther. 2019 Oct 15;11:306-315
pubmed: 31687424
J Craniofac Surg. 2018 May;29(3):741-746
pubmed: 29309349
N Engl J Med. 1994 Oct 6;331(14):889-95
pubmed: 8078550
Biomed Res Int. 2014;2014:272481
pubmed: 25210707
Arch Orthop Trauma Surg. 2000;120(3-4):121-7
pubmed: 10738867
Tissue Eng. 2005 Sep-Oct;11(9-10):1421-8
pubmed: 16259597
Life Sci. 1975 Dec 1;17(11):1721-32
pubmed: 1207385
Cell. 1982 Aug;30(1):215-24
pubmed: 7127471
J Bone Joint Surg Am. 2010 Sep 15;92(12):2220-33
pubmed: 20844166
Clin Orthop Relat Res. 2004 Mar;(420):276-85
pubmed: 15057109
J Orthop Res. 2005 Mar;23(2):425-32
pubmed: 15734258
Regen Ther. 2017 Oct 09;7:72-79
pubmed: 30271854
Nat Med. 2010 Jul;16(7):814-20
pubmed: 20543851
Plast Surg Int. 2012;2012:562892
pubmed: 23213503
Nat Med. 2008 Feb;14(2):213-21
pubmed: 18193059
Biochem J. 1986 Dec 1;240(2):423-30
pubmed: 3545187
Tissue Eng Part A. 2009 May;15(5):1109-18
pubmed: 19061429
Acta Biomater. 2009 Feb;5(2):670-9
pubmed: 18951858
Annu Rev Cell Dev Biol. 2006;22:287-309
pubmed: 16824016
Knee Surg Sports Traumatol Arthrosc. 2010 Oct;18(10):1400-6
pubmed: 20033674
Plast Reconstr Surg. 2008 Jun;121(6):1949-1955
pubmed: 18520880
Biomaterials. 2020 Mar;235:119821
pubmed: 32006743
Acta Biomater. 2006 May;2(3):313-20
pubmed: 16701890
Indian J Orthop. 2016 Jul-Aug;50(4):414-20
pubmed: 27512224
Stem Cell Res. 2010 Jan;4(1):57-68
pubmed: 19880363
Tissue Eng. 2007 Jul;13(7):1583-92
pubmed: 17630901
Matrix Biol. 2010 Oct;29(8):690-700
pubmed: 20797438
J Int Adv Otol. 2019 Aug;15(2):204-208
pubmed: 31418720
J Orthop Res. 2004 Mar;22(2):446-55
pubmed: 15013108
J Cell Physiol. 2018 Feb;233(2):1490-1499
pubmed: 28574571
Acta Biomater. 2009 Jan;5(1):1-13
pubmed: 18938117
Biomaterials. 2011 Apr;32(12):3233-43
pubmed: 21296410
J Biomed Mater Res. 2000 Aug;51(2):273-9
pubmed: 10825227
J Biomed Mater Res A. 2006 Jul;78(1):1-11
pubmed: 16596585
J Bone Joint Surg Br. 2009 May;91(5):565-76
pubmed: 19407287
Biochem J. 1989 Jun 1;260(2):543-8
pubmed: 2788408