Isolation of Proteins from Extracellular Vesicles (EVs) for Mass Spectrometry-Based Proteomic Analyses.
Data-dependent acquisition
Exosomes
Extracellular vesicles (EVs)
Mass spectrometry
Proteomics
Sample preparation
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
9
1
2021
pubmed:
10
1
2021
medline:
1
4
2021
Statut:
ppublish
Résumé
Extracellular vesicles (EVs) are freely circulating nano/micrometer-sized membrane-bound vesicles released by various cell types. Their cargo consists of proteins, lipids, metabolites, and different types of RNA molecules reflecting the origin of the secreting cell type or tissue. Since the EV cargo is constantly changing in response to pathological status or different environmental stressors, it has been extensively studied in the quest for biomarkers, especially in the cancer research. Mass spectrometry (MS)-based proteome analysis is a powerful tool to elucidate the protein cargo in EVs. This chapter describes and characterizes three MS-compatible lysis methods, namely by using urea, guanidium hydrochloride, and radioimmunoprecipitation buffer for isolating proteins from EVs.
Identifiants
pubmed: 33420991
doi: 10.1007/978-1-0716-1186-9_12
doi:
Substances chimiques
Proteins
0
Urea
8W8T17847W
Guanidine
JU58VJ6Y3B
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
207-212Références
Maas SLN, Breakefield XO, Weaver AM (2017) Extracellular vesicles: unique intercellular delivery vehicles. Trends Cell Biol 27:172–188
doi: 10.1016/j.tcb.2016.11.003
van NG, D’Angelo G, Raposo G (2018) Shedding light on the cell biology of extracellular vesicles. Nat Rev Mol Cell Biol 19:213–228
doi: 10.1038/nrm.2017.125
Atkin-Smith GK, Poon IKH (2017) Disassembly of the dying: mechanisms and functions. Trends Cell Biol 27:151–162
doi: 10.1016/j.tcb.2016.08.011
Skotland T, Sandvig K, Llorente A (2017) Lipids in exosomes: current knowledge and the way forward. Prog Lipid Res 66:30–41
doi: 10.1016/j.plipres.2017.03.001
Puhka M, Takatalo M, Nordberg ME et al (2017) Metabolomic profiling of extracellular vesicles and alternative normalization methods reveal enriched metabolites and strategies to study prostate cancer-related changes. Theranostics 7:3824–3841
doi: 10.7150/thno.19890
Yentrapalli R, Merl-Pham J, Azimzadeh O et al (2017) Quantitative changes in the protein and miRNA cargo of plasma exosome-like vesicles after exposure to ionizing radiation. Int J Radiat Biol 93:569–580
doi: 10.1080/09553002.2017.1294772
Subedi P, Schneider M, Philipp J et al (2019) Comparison of methods to isolate proteins from extracellular vesicles for mass spectrometry-based proteomic analyses. Anal Biochem 584:113390
doi: 10.1016/j.ab.2019.113390
Yáñez-Mó M, Siljander PRM, Andreu Z et al (2015) Biological properties of extracellular vesicles and their physiological functions. J Extracell Vesicles 4:1–60
doi: 10.3402/jev.v4.27066
Colombo M, Raposo G, Théry C (2014) Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles. Annu Rev Cell Dev Biol 30:255–289
doi: 10.1146/annurev-cellbio-101512-122326
Caby MP, Lankar D, Vincendeau-Scherrer C et al (2005) Exosomal-like vesicles are present in human blood plasma. Int Immunol 17:879–887
doi: 10.1093/intimm/dxh267
Ogawa Y, Miura Y, Harazono A et al (2011) Proteomic analysis of two types of exosomes in human whole saliva. Biol Pharm Bull 34:13–23
doi: 10.1248/bpb.34.13
Kong F-L, Wang X-P, Li Y-N et al (2018) The role of exosomes derived from cerebrospinal fluid of spinal cord injury in neuron proliferation in vitro. Artif Cells Nanomed Biotechnol 46:200–205
doi: 10.1080/21691401.2017.1304408
Ratajczak J, Wysoczynski M, Hayek F et al (2006) Membrane-derived microvesicles: Important and underappreciated mediators of cell-to-cell communication. Leukemia 20:1487–1495
doi: 10.1038/sj.leu.2404296
Lim Y-J, Lee S-J (2017) Are exosomes the vehicle for protein aggregate propagation in neurodegenerative diseases? Acta Neuropathol Commun 5:64
doi: 10.1186/s40478-017-0467-z
Panigrahi GK, Praharaj PP, Kittaka H et al (2019) Exosome proteomic analyses identify inflammatory phenotype and novel biomarkers in African American prostate cancer patients. Cancer Med 8:1110–1123
doi: 10.1002/cam4.1885
Rabilloud T (2002) Protein solubility in two-dimensional electrophoresis. In: Walker JM (ed) The protein protocols handbook. Humana Press, Totowa, NJ, pp 131–140
doi: 10.1385/1-59259-169-8:131
Théry C, Aled C, Sebastian A et al (2006) Isolation and characterization of exosomes from cell culture supernatants. Curr Protoc Cell Biol 3(22):1–29
Wiśniewski JR, Zougman A, Nagaraj N et al (2009) Universal sample preparation method for proteome analysis. Nat Methods 6:359–362
doi: 10.1038/nmeth.1322