Imaging the local biochemical content of native and injured intervertebral disc using Fourier transform infrared microscopy.
Fourier transform infrared microscopy
collagen
degeneration
intervertebral disc
proteoglycan
Journal
JOR spine
ISSN: 2572-1143
Titre abrégé: JOR Spine
Pays: United States
ID NLM: 101722350
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
06
02
2020
revised:
20
07
2020
accepted:
03
08
2020
entrez:
4
1
2021
pubmed:
5
1
2021
medline:
5
1
2021
Statut:
epublish
Résumé
Alterations to the biochemical composition of the intervertebral disc (IVD) are hallmarks of aging and degeneration. Methods to assess biochemical content, such as histology, immunohistochemistry, and spectrophotometric assays, are limited in their ability to quantitatively analyze the spatial distribution of biochemical components. Fourier transform infrared (FTIR) microscopy is a biochemical analysis method that can yield both quantitative and high-resolution data about the spatial distribution of biochemical components. This technique has been largely unexplored for use with the IVD, and existing methods use complex analytical techniques that make results difficult to interpret. The objective of the present study is to describe an FTIR microscopy method that has been optimized for imaging the collagen and proteoglycan content of the IVD. The method was performed on intact and discectomized IVDs from the sheep lumbar spine after 6 weeks in vivo in order to validate FTIR microscopy in healthy and degenerated IVDs. FTIR microscopy quantified collagen and proteoglycan content across the entire IVD and showed local changes in biochemical content after discectomy that were not observed with traditional histological methods. Changes in collagen and proteoglycans content were found to have strong correlations with Pfirrmann grades of degeneration. This study demonstrates how FTIR microscopy is a valuable research tool that can be used to quantitatively assess the local biochemical composition of IVDs in development, degeneration, and repair.
Identifiants
pubmed: 33392456
doi: 10.1002/jsp2.1121
pii: JSP21121
pmc: PMC7770196
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e1121Informations de copyright
© 2020 The Authors. JOR Spine published by Wiley Periodicals LLC on behalf of Orthopaedic Research Society.
Déclaration de conflit d'intérêts
Roger Härtl is a consultant for AO Spine, Brainlab, Depuy‐Synthes and Lanx, and received research funding from Baxter. Lawrence J. Bonassar is a consultant for Fidia Pharmaceuticals and 3DBio Corp.
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