Transcription Factor Pit-1 Affects Transcriptional Timing in the Dual-Promoter Human Prolactin Gene.
Pit-1
gene transcription
pituitary
prolactin gene
promoter
transcription timing
Journal
Endocrinology
ISSN: 1945-7170
Titre abrégé: Endocrinology
Pays: United States
ID NLM: 0375040
Informations de publication
Date de publication:
01 04 2021
01 04 2021
Historique:
received:
26
11
2020
pubmed:
4
1
2021
medline:
21
10
2021
entrez:
3
1
2021
Statut:
ppublish
Résumé
Gene transcription occurs in short bursts interspersed with silent periods, and these kinetics can be altered by promoter structure. The effect of alternate promoter architecture on transcription bursting is not known. We studied the human prolactin (hPRL) gene that contains 2 promoters, a pituitary-specific promoter that requires the transcription factor Pit-1 and displays dramatic transcriptional bursting activity and an alternate upstream promoter that is active in nonpituitary tissues. We studied large hPRL genomic fragments with luciferase reporters, and used bacterial artificial chromosome recombineering to manipulate critical promoter regions. Stochastic switch mathematical modelling of single-cell time-lapse luminescence image data revealed that the Pit-1-dependent promoter showed longer, higher-amplitude transcriptional bursts. Knockdown studies confirmed that the presence of Pit-1 stabilized and prolonged periods of active transcription. Pit-1 therefore plays an active role in establishing the timing of transcription cycles, in addition to its cell-specific functions.
Identifiants
pubmed: 33388754
pii: 6060060
doi: 10.1210/endocr/bqaa249
pmc: PMC7871365
pii:
doi:
Substances chimiques
Transcription Factor Pit-1
0
Prolactin
9002-62-4
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/K003097/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 091688
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 091688
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/F01760X/1
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/K015885/1
Pays : United Kingdom
Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of the Endocrine Society.
Références
PLoS One. 2009 Sep 03;4(9):e6845
pubmed: 19727442
Genes Dev. 1990 May;4(5):695-711
pubmed: 2379827
Science. 2003 Feb 21;299(5610):1183-4
pubmed: 12595674
J Neuroendocrinol. 2012 Jul;24(7):977-90
pubmed: 22420298
Cell. 2014 Jul 17;158(2):314-326
pubmed: 25036631
Nature. 1998 Sep 17;395(6699):301-6
pubmed: 9751061
Nat Rev Genet. 2013 Aug;14(8):572-84
pubmed: 23835438
Mol Cell. 2015 Apr 2;58(1):147-56
pubmed: 25728770
Curr Opin Endocrinol Diabetes Obes. 2008 Aug;15(4):359-63
pubmed: 18594277
Endocrinology. 2012 Jun;153(6):2724-34
pubmed: 22495675
Endocrinology. 2001 Jul;142(7):3255-60
pubmed: 11416049
J Cell Biochem. 1999 Mar 1;72(3):322-38
pubmed: 10022514
Mol Endocrinol. 1994 Mar;8(3):356-73
pubmed: 8015553
J Cell Sci. 2010 Feb 1;123(Pt 3):424-30
pubmed: 20130141
Mol Endocrinol. 1994 May;8(5):635-42
pubmed: 8058071
Nat Genet. 2002 Jan;30(1):29-30
pubmed: 11743582
Endocrinology. 2008 Feb;149(2):687-94
pubmed: 18006630
PLoS Comput Biol. 2016 Aug 23;12(8):e1005072
pubmed: 27551778
Mol Biosyst. 2017 Jun 27;13(7):1280-1290
pubmed: 28573295
Biostatistics. 2015 Oct;16(4):655-69
pubmed: 25819987
Mol Endocrinol. 2009 Apr;23(4):529-38
pubmed: 19147700
Mol Cell Biol. 2013 May;33(9):1723-34
pubmed: 23428872
Mol Cell Endocrinol. 1991 Sep;80(1-3):53-64
pubmed: 1955081
Cell Syst. 2017 Dec 27;5(6):646-653.e5
pubmed: 29153839
Mol Endocrinol. 2005 Jan;19(1):138-47
pubmed: 15375187
Endocrinology. 2021 Apr 1;162(4):
pubmed: 33388754
Endocr Rev. 2008 Feb;29(1):1-41
pubmed: 18057139
Cell Rep. 2015 Nov 10;13(6):1284-1285
pubmed: 28873343
Endocrinology. 1998 Mar;139(3):1361-8
pubmed: 9492073
Mol Endocrinol. 1991 Nov;5(11):1748-54
pubmed: 1779976
Endocr Rev. 1998 Jun;19(3):225-68
pubmed: 9626554
Science. 2009 Apr 10;324(5924):242-6
pubmed: 19359585
Proc Natl Acad Sci U S A. 2013 Dec 17;110(51):20563-8
pubmed: 24297917
Mol Endocrinol. 2014 May;28(5):622-33
pubmed: 24694306
Proc Natl Acad Sci U S A. 2012 Oct 23;109(43):17454-9
pubmed: 23064634
J Biol Chem. 1993 Nov 5;268(31):23366-75
pubmed: 8226861
Science. 2011 Apr 22;332(6028):472-4
pubmed: 21415320
Genes Dev. 1989 Jul;3(7):946-58
pubmed: 2550324
Nucleic Acids Res. 2005 Feb 24;33(4):e36
pubmed: 15731329
Genes Dev. 1991 Jul;5(7):1309-20
pubmed: 2065979
PLoS Biol. 2011 Apr;9(4):e1000607
pubmed: 21532732
Mol Endocrinol. 2016 Feb;30(2):189-200
pubmed: 26691151
J Endocrinol. 2010 Jul;206(1):1-11
pubmed: 20371569
Mol Endocrinol. 1991 Oct;5(10):1488-97
pubmed: 1775132
PLoS One. 2010 Aug 18;5(8):e12274
pubmed: 20806066
Elife. 2016 Feb 01;5:e08494
pubmed: 26828110
Bioessays. 2006 Oct;28(10):1051-5
pubmed: 16998840
Cell Syst. 2018 Apr 25;6(4):409-423.e11
pubmed: 29454937
Cell. 1988 Nov 4;55(3):519-29
pubmed: 2902928
Mol Syst Biol. 2015 May 05;11(5):806
pubmed: 25943345
Acta Paediatr Suppl. 1999 Dec;88(433):33-41
pubmed: 10626543
J Cell Sci. 2011 Oct 15;124(Pt 20):3484-91
pubmed: 21984812
Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10598-603
pubmed: 24994903
Mol Syst Biol. 2015 Jul 27;11(7):823
pubmed: 26215071