Potential role for age as a modulator of oral nitrate reductase activity.
Aging
Microbiome
Nitric oxide
Journal
Nitric oxide : biology and chemistry
ISSN: 1089-8611
Titre abrégé: Nitric Oxide
Pays: United States
ID NLM: 9709307
Informations de publication
Date de publication:
01 03 2021
01 03 2021
Historique:
received:
11
09
2020
revised:
07
12
2020
accepted:
09
12
2020
pubmed:
16
12
2020
medline:
17
8
2021
entrez:
15
12
2020
Statut:
ppublish
Résumé
Reduction of salivary nitrate to nitrite by oral nitrate reductase (NR) expressing bacteria has emerged as an integral pathway in regulating nitric oxide (NO) homeostasis and signaling. The oral microbiome is critical for this pathway. Variations in this pathway may underlie variable responses in the magnitude by which dietary or therapeutic nitrate modulates NO-signaling. The relationships between oral microbes and NR activity, and the factors that affect this relationship remain unclear however. Using a cross-sectional study design, the objective of this study was to determine the relationships between oral microbes and oral NR activity using a protocol that directly measures initial NR activity. Tongue swabs were collected from 28 subjects ranging in age from 21 to 73y. Initial NR activity showed a bell-shaped dependence with age, with activity peaking at ~40-50y and being lower but similar between younger (20-30y) and older (51-73) individuals. Microbiome relative abundance and diversity analyses, using 16s sequencing, demonstrated differences across age and identified both NR expressing and non-expressing bacteria in modulating initial NR activity. Finally, initial NR activity was measured in 3mo and 13mo old C57BL/6J mice. No differences in bacterial number were observed. However initial NR activity was significantly (80%) lower in 13mo old mice. Collectively, these data suggest that age is a variable in NR activity and may modulate responsiveness to dietary nitrate.
Identifiants
pubmed: 33321206
pii: S1089-8603(20)30212-3
doi: 10.1016/j.niox.2020.12.001
pmc: PMC8085911
mid: NIHMS1695484
pii:
doi:
Substances chimiques
Bacterial Proteins
0
Nitrates
0
Nitrites
0
Nitrate Reductase
EC 1.7.99.4
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1-7Subventions
Organisme : NIDDK NIH HHS
ID : P30 DK079626
Pays : United States
Organisme : NHLBI NIH HHS
ID : T32 HL007457
Pays : United States
Organisme : NIDCR NIH HHS
ID : T90 DE022736
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR001417
Pays : United States
Commentaires et corrections
Type : ErratumIn
Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Références
Hypertension. 2015 Feb;65(2):320-7
pubmed: 25421976
Hypertension. 2008 Mar;51(3):784-90
pubmed: 18250365
J Appl Physiol (1985). 2009 Oct;107(4):1144-55
pubmed: 19661447
Circulation. 2010 Jan 5;121(1):98-109
pubmed: 20026772
Mol Nutr Food Res. 2016 Jan;60(1):185-202
pubmed: 26256112
Nat Rev Drug Discov. 2015 Sep;14(9):623-41
pubmed: 26265312
N Engl J Med. 2006 Dec 28;355(26):2792-3
pubmed: 17192551
Cardiovasc Res. 2011 Feb 15;89(3):507-15
pubmed: 20952416
Nitric Oxide. 2012 May 15;26(4):197-202
pubmed: 22285857
Nitric Oxide. 2017 Jun 1;66:62-70
pubmed: 28390999
Appl Environ Microbiol. 1997 Mar;63(3):924-30
pubmed: 9055411
Am J Physiol Heart Circ Physiol. 2009 May;296(5):H1281-8
pubmed: 19252084
Appl Physiol Nutr Metab. 2014 Sep;39(9):1019-28
pubmed: 25068792
Pediatr Res. 2015 Jan;77(1-2):173-81
pubmed: 25314582
Nitric Oxide. 2012 Dec 1;27(4):193-200
pubmed: 22842223
Proc Natl Acad Sci U S A. 2007 Nov 27;104(48):19144-9
pubmed: 18025468
Nat Rev Drug Discov. 2008 Feb;7(2):156-67
pubmed: 18167491
J Clin Invest. 2005 May;115(5):1232-40
pubmed: 15841216
Nitric Oxide. 2020 Jan 1;94:73-78
pubmed: 31682925
Nitric Oxide. 2019 Aug 1;89:54-63
pubmed: 31051259
PLoS One. 2014 Mar 26;9(3):e88645
pubmed: 24670812
Nat Chem Biol. 2005 Oct;1(5):290-7
pubmed: 16408059
Free Radic Biol Med. 2013 Feb;55:93-100
pubmed: 23183324
Nutrients. 2019 Jul 22;11(7):
pubmed: 31336633
Free Radic Biol Med. 2018 Aug 20;124:21-30
pubmed: 29807159
Am J Physiol Heart Circ Physiol. 2018 Jan 1;314(1):H45-H51
pubmed: 28971842
J Clin Invest. 2007 Sep;117(9):2583-91
pubmed: 17717604
Free Radic Biol Med. 2013 Dec;65:1521-1532
pubmed: 23806384
PLoS One. 2014 Feb 14;9(2):e89398
pubmed: 24551246
Nitric Oxide. 2014 Apr 30;38:45-57
pubmed: 24650698
Eur J Sport Sci. 2019 Feb;19(1):15-29
pubmed: 29529987
Nitric Oxide. 2011 Jan 1;24(1):34-42
pubmed: 20951824
Free Radic Biol Med. 2018 Oct;126:113-121
pubmed: 30031863
Eur J Oral Sci. 2004 Oct;112(5):424-8
pubmed: 15458501
Hypertension. 2010 Aug;56(2):274-81
pubmed: 20585108
Comp Biochem Physiol A Physiol. 1997 Dec;118(4):939-48
pubmed: 9505412
Blood. 2006 Jan 15;107(2):566-74
pubmed: 16195332
Am J Physiol Heart Circ Physiol. 2006 Nov;291(5):H2026-35
pubmed: 16798825
Nutr Res. 2015 Aug;35(8):643-54
pubmed: 26189149
Free Radic Biol Med. 2013 Jul;60:89-97
pubmed: 23395779
Eur J Appl Physiol. 2016 Feb;116(2):415-25
pubmed: 26614506
Nat Chem Biol. 2009 Dec;5(12):865-9
pubmed: 19915529
Nitric Oxide. 2017 Dec 1;71:14-20
pubmed: 28939409
Vasc Cell. 2014 Sep 16;6:19
pubmed: 25243060
Eur J Oral Sci. 2005 Feb;113(1):14-9
pubmed: 15693824
Nutrients. 2017 Oct 27;9(11):
pubmed: 29077028
Am J Clin Nutr. 2016 Jan;103(1):25-38
pubmed: 26607938
J Intern Med. 2016 Apr;279(4):315-36
pubmed: 26522443
Front Cell Infect Microbiol. 2019 Mar 01;9:39
pubmed: 30881924
Circulation. 2012 Oct 16;126(16):1983-92
pubmed: 22992322
Curr Protoc Hum Genet. 2014 Jul 14;82:18.8.1-29
pubmed: 25042718
Eur J Nutr. 2016 Mar;55(2):451-459
pubmed: 25764393
Free Radic Biol Med. 2018 May 20;120:80-88
pubmed: 29550328
Free Radic Biol Med. 2014 Dec;77:249-57
pubmed: 25305639
Nat Rev Microbiol. 2004 Jul;2(7):593-602
pubmed: 15197394
Nitric Oxide. 2019 Feb 1;83:1-10
pubmed: 30528912
Blood Press. 2020 Apr;29(2):103-112
pubmed: 31709856
Curr Opin Lipidol. 2011 Feb;22(1):11-5
pubmed: 21102328
Free Radic Biol Med. 2019 Jul;138:63-72
pubmed: 31082507