Biofilm-coated microbeads and the mouse ear skin: An innovative model for analysing anti-biofilm immune response in vivo.
Animals
Biofilms
/ growth & development
Cell Movement
Disease Models, Animal
Ear Auricle
/ immunology
Female
Fluorescent Dyes
/ chemistry
Immunity, Innate
Male
Mice
Mice, Inbred C57BL
Mice, Transgenic
Microscopy, Confocal
Microspheres
Phagocytes
/ cytology
Silicon Dioxide
/ chemistry
Staphylococcal Infections
/ immunology
Staphylococcus aureus
/ pathogenicity
Time-Lapse Imaging
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
19
08
2020
accepted:
20
11
2020
entrez:
4
12
2020
pubmed:
5
12
2020
medline:
28
1
2021
Statut:
epublish
Résumé
Owing to its ability to form biofilms, Staphylococcus aureus is responsible for an increasing number of infections on implantable medical devices. The aim of this study was to develop a mouse model using microbeads coated with S. aureus biofilm to simulate such infections and to analyse the dynamics of anti-biofilm inflammatory responses by intravital imaging. Scanning electron microscopy and flow cytometry were used in vitro to study the ability of an mCherry fluorescent strain of S. aureus to coat silica microbeads. Biofilm-coated microbeads were then inoculated intradermally into the ear tissue of LysM-EGFP transgenic mice (EGFP fluorescent immune cells). General and specific real-time inflammatory responses were studied in ear tissue by confocal microscopy at early (4-6h) and late time points (after 24h) after injection. The displacement properties of immune cells were analysed. The responses were compared with those obtained in control mice injected with only microbeads. In vitro, our protocol was capable of generating reproducible inocula of biofilm-coated microbeads verified by labelling matrix components, observing biofilm ultrastructure and confirmed in vivo and in situ with a matrix specific fluorescent probe. In vivo, a major inflammatory response was observed in the mouse ear pinna at both time points. Real-time observations of cell recruitment at injection sites showed that immune cells had difficulty in accessing biofilm bacteria and highlighted areas of direct interaction. The average speed of cells was lower in infected mice compared to control mice and in tissue areas where direct contact between immune cells and bacteria was observed, the average cell velocity and linearity were decreased in comparison to cells in areas where no bacteria were visible. This model provides an innovative way to analyse specific immune responses against biofilm infections on medical devices. It paves the way for live evaluation of the effectiveness of immunomodulatory therapies combined with antibiotics.
Identifiants
pubmed: 33275636
doi: 10.1371/journal.pone.0243500
pii: PONE-D-20-26002
pmc: PMC7717515
doi:
Substances chimiques
Fluorescent Dyes
0
Silicon Dioxide
7631-86-9
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0243500Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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