Identification of SARS-CoV-2 inhibitors using lung and colonic organoids.
Animals
Antiviral Agents
/ pharmacology
COVID-19
/ prevention & control
Colon
/ cytology
Drug Approval
Drug Evaluation, Preclinical
/ methods
Female
Heterografts
/ drug effects
Humans
In Vitro Techniques
Lung
/ cytology
Male
Mice
Organoids
/ cytology
SARS-CoV-2
/ drug effects
United States
United States Food and Drug Administration
Viral Tropism
Virus Internalization
/ drug effects
COVID-19 Drug Treatment
Journal
Nature
ISSN: 1476-4687
Titre abrégé: Nature
Pays: England
ID NLM: 0410462
Informations de publication
Date de publication:
01 2021
01 2021
Historique:
received:
05
05
2020
accepted:
21
10
2020
pubmed:
30
10
2020
medline:
23
1
2021
entrez:
29
10
2020
Statut:
ppublish
Résumé
There is an urgent need to create novel models using human disease-relevant cells to study severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) biology and to facilitate drug screening. Here, as SARS-CoV-2 primarily infects the respiratory tract, we developed a lung organoid model using human pluripotent stem cells (hPSC-LOs). The hPSC-LOs (particularly alveolar type-II-like cells) are permissive to SARS-CoV-2 infection, and showed robust induction of chemokines following SARS-CoV-2 infection, similar to what is seen in patients with COVID-19. Nearly 25% of these patients also have gastrointestinal manifestations, which are associated with worse COVID-19 outcomes
Identifiants
pubmed: 33116299
doi: 10.1038/s41586-020-2901-9
pii: 10.1038/s41586-020-2901-9
pmc: PMC8034380
mid: NIHMS1640339
doi:
Substances chimiques
Antiviral Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
270-275Subventions
Organisme : NIDDK NIH HHS
ID : DP3 DK111907
Pays : United States
Organisme : NCI NIH HHS
ID : R00 CA226353
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK119667
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK124463
Pays : United States
Organisme : NIDDK NIH HHS
ID : R03 DK117252
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK116075
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK121072
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL105947
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI107301
Pays : United States
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