Establishment and Long-Term Culture of Organoids Derived from Human Biliary Tract Carcinoma.
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
19 06 2020
19 06 2020
Historique:
entrez:
28
10
2020
pubmed:
29
10
2020
medline:
29
10
2020
Statut:
epublish
Résumé
This protocol is a procedure for establishment and culture of cancer and non-cancer organoids using tissues from biliary tract carcinoma (BTC) patients. These BTC organoids can be used for various biological analyses and drug screening. One challenge in establishing and culturing BTC organoids is non-cancer cells contaminating surgically resected tumor tissues form organoids concurrently with cancer organoids. Careful validation that the established organoids are cancer-derived is important. For complete details on the use and generation of this protocol, please refer to Saito et al. (2019) in the journal
Identifiants
pubmed: 33111070
doi: 10.1016/j.xpro.2019.100009
pii: S2666-1667(19)30009-7
pmc: PMC7580121
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
100009Informations de copyright
© 2020.
Déclaration de conflit d'intérêts
The authors declare no competing interests.
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