Ultra-sensitive viral glycoprotein detection NanoSystem toward accurate tracing SARS-CoV-2 in biological/non-biological media.
Animals
Betacoronavirus
/ isolation & purification
Biosensing Techniques
/ instrumentation
COVID-19
COVID-19 Testing
Clinical Laboratory Techniques
Coronavirus Infections
/ diagnosis
Electrochemical Techniques
/ instrumentation
Electrodes
Equipment Design
Gold
/ chemistry
Graphite
/ chemistry
Humans
Limit of Detection
Metal Nanoparticles
/ chemistry
Pandemics
Pneumonia, Viral
/ diagnosis
SARS-CoV-2
Spike Glycoprotein, Coronavirus
/ analysis
Electrochemical detection
Nanosensor
Outbreak
SARS-CoV-2
Virus
Journal
Biosensors & bioelectronics
ISSN: 1873-4235
Titre abrégé: Biosens Bioelectron
Pays: England
ID NLM: 9001289
Informations de publication
Date de publication:
01 Jan 2021
01 Jan 2021
Historique:
received:
07
08
2020
revised:
09
10
2020
accepted:
12
10
2020
pubmed:
20
10
2020
medline:
27
11
2020
entrez:
19
10
2020
Statut:
ppublish
Résumé
Rapid person-to-person transfer of viruses such as SARS-CoV-2 and their occasional mutations owing to the human activity and climate/ecological changes by the mankind led to creation of wrecking worldwide challenges. Such fast transferable pathogens requiring practical diagnostic setups to control their transfer chain and stop sever outbreaks in early stages of their appearance. Herein, we have addressed this urgent demand by designing a rapid electrochemical diagnostic kit composed of fixed/screen printed electrodes that can detect pathogenic viruses such as SARS-CoV-2 and/or animal viruses through the differentiable fingerprint of their viral glycoproteins at different voltage positions. The working electrode of developed sensor is activated upon coating a layer of coupled graphene oxide (GO) with sensitive chemical compounds along with gold nanostars (Au NS) that can detect the trace of viruses in any aquatic biological media (e.g., blood, saliva and oropharyngeal/nasopharyngeal swab) through interaction with active functional groups of their glycoproteins. The method do not require any extraction and/or biomarkers for detection of target viruses and can identify trace of different pathogenic viruses in about 1 min. The nanosensor also demonstrated superior limit of detection (LOD) and sensitivity of 1.68 × 10
Identifiants
pubmed: 33075725
pii: S0956-5663(20)30719-3
doi: 10.1016/j.bios.2020.112731
pmc: PMC7558249
pii:
doi:
Substances chimiques
Spike Glycoprotein, Coronavirus
0
graphene oxide
0
spike protein, SARS-CoV-2
0
Gold
7440-57-5
Graphite
7782-42-5
Types de publication
Evaluation Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
112731Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Références
Lancet. 2020 Feb 15;395(10223):514-523
pubmed: 31986261
Sci Rep. 2020 Mar 4;10(1):4033
pubmed: 32132578
Anal Chem. 2015 Dec 1;87(23):11691-9
pubmed: 26536155
Clin Chem. 2005 Dec;51(12):2415-8
pubmed: 16179424
Angew Chem Int Ed Engl. 2014 Sep 22;53(39):10386-9
pubmed: 25088356
Emerg Infect Dis. 2005 Dec;11(12):1815-21
pubmed: 16485464
Angew Chem Int Ed Engl. 2014 Nov 10;53(46):12489-93
pubmed: 25214064
ACS Appl Mater Interfaces. 2015 Jul 29;7(29):16011-7
pubmed: 26161682
Sci China Life Sci. 2020 Mar;63(3):457-460
pubmed: 32009228
Science. 2001 Mar 23;291(5512):2370-6
pubmed: 11269318
Chem Rev. 2013 Apr 10;113(4):2668-732
pubmed: 23531120
Chem Soc Rev. 2016 Apr 21;45(8):2137-211
pubmed: 26936282
Mater Today Chem. 2020 Sep;17:100306
pubmed: 32835155
Analyst. 2013 Nov 7;138(21):6219-29
pubmed: 24043121
Anal Chim Acta. 2015 May 4;872:84-94
pubmed: 25892073
Chem Rev. 2015 Mar 11;115(5):2045-108
pubmed: 25659975
J Chromatogr A. 2016 Dec 2;1475:102-109
pubmed: 27863712
Avian Pathol. 2012;41(3):251-8
pubmed: 22702452