Effect of DNMT3A polymorphisms on CpG island hypermethylation in gastric mucosa.
Aged
Alleles
Antigens, CD
/ genetics
Cadherins
/ genetics
CpG Islands
/ genetics
Cyclin-Dependent Kinase Inhibitor p16
/ genetics
DNA (Cytosine-5-)-Methyltransferases
/ genetics
DNA Methylation
DNA Methyltransferase 3A
Death-Associated Protein Kinases
/ genetics
Female
Gastric Mucosa
/ metabolism
Gene Frequency
Genotype
Helicobacter Infections
/ diagnosis
Helicobacter pylori
/ physiology
Humans
Male
Middle Aged
Polymorphism, Single Nucleotide
CpG island
DNMT3A
Gastric mucosa
Genetic polymorphism
Hypermethylation
Journal
BMC medical genetics
ISSN: 1471-2350
Titre abrégé: BMC Med Genet
Pays: England
ID NLM: 100968552
Informations de publication
Date de publication:
16 10 2020
16 10 2020
Historique:
received:
29
06
2020
accepted:
08
10
2020
entrez:
17
10
2020
pubmed:
18
10
2020
medline:
3
11
2020
Statut:
epublish
Résumé
CpG methylation of tumor suppressor genes occurs in the early stage of carcinogenesis. Detecting risk factors for aberrant CpG methylation is clinically important for predicting cancer development. DNA methyltransferase (DNMT) 3a is considered to play critical roles in the DNA methylation process during pathogenesis. In this study, we evaluated the association between DNMT3A polymorphisms (rs6733868 and rs13428812) and CpG methylation status in non-cancerous gastric mucosa. We determined the DNMT3A genotype and CpG methylation status of 4 genes (p14 The minor allele frequencies of both polymorphisms (rs6733868 and rs13428812) were lower in the CpG methylated groups of each of the 4 genes (p14 Our study indicates that polymorphisms of DNMT3A are associated with the accumulation of gene methylation in gastric mucosa. Carrying the minor alleles of rs6733868 or rs13428812 inhibits aberrant gene methylations, which are typically enhanced by HP infection.
Sections du résumé
BACKGROUND
CpG methylation of tumor suppressor genes occurs in the early stage of carcinogenesis. Detecting risk factors for aberrant CpG methylation is clinically important for predicting cancer development. DNA methyltransferase (DNMT) 3a is considered to play critical roles in the DNA methylation process during pathogenesis. In this study, we evaluated the association between DNMT3A polymorphisms (rs6733868 and rs13428812) and CpG methylation status in non-cancerous gastric mucosa.
METHODS
We determined the DNMT3A genotype and CpG methylation status of 4 genes (p14
RESULTS
The minor allele frequencies of both polymorphisms (rs6733868 and rs13428812) were lower in the CpG methylated groups of each of the 4 genes (p14
CONCLUSIONS
Our study indicates that polymorphisms of DNMT3A are associated with the accumulation of gene methylation in gastric mucosa. Carrying the minor alleles of rs6733868 or rs13428812 inhibits aberrant gene methylations, which are typically enhanced by HP infection.
Identifiants
pubmed: 33066747
doi: 10.1186/s12881-020-01142-7
pii: 10.1186/s12881-020-01142-7
pmc: PMC7562764
doi:
Substances chimiques
Antigens, CD
0
CDH1 protein, human
0
Cadherins
0
Cyclin-Dependent Kinase Inhibitor p16
0
DNMT3A protein, human
0
DNA (Cytosine-5-)-Methyltransferases
EC 2.1.1.37
DNA Methyltransferase 3A
EC 2.1.1.37
Death-Associated Protein Kinases
EC 2.7.11.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
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