Clostridioides difficile carriage in animals and the associated changes in the host fecal microbiota.
Animals
Anti-Bacterial Agents
/ pharmacology
Bacterial Toxins
/ genetics
Bacterial Typing Techniques
Cats
Chlorocebus aethiops
Clostridioides difficile
/ classification
Clostridium Infections
/ epidemiology
Coculture Techniques
Dogs
Feces
/ microbiology
Female
Gastrointestinal Microbiome
Horses
Hospitals, Animal
Host-Pathogen Interactions
Male
Microbial Interactions
Microbial Sensitivity Tests
North Carolina
Polymerase Chain Reaction
Prevalence
RNA, Ribosomal, 16S
Ribotyping
Risk Factors
Tertiary Healthcare
Vero Cells
Animal
Antibiotic resistance
C. difficile
C. hiranonis
Canine
Equine
Feline
Microbiome
Ribotype
Journal
Anaerobe
ISSN: 1095-8274
Titre abrégé: Anaerobe
Pays: England
ID NLM: 9505216
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
03
03
2020
revised:
31
08
2020
accepted:
27
09
2020
pubmed:
7
10
2020
medline:
14
8
2021
entrez:
6
10
2020
Statut:
ppublish
Résumé
The relationship between the gut microbiota and Clostridioides difficile, and its role in the severity of C. difficile infection in humans is an area of active research. Intestinal carriage of toxigenic and non-toxigenic C. difficile strains, with and without clinical signs, is reported in animals, however few studies have looked at the risk factors associated with C. difficile carriage and the role of the host gut microbiota. Here, we isolated and characterized C. difficile strains from different animal species (predominantly canines (dogs), felines (cats), and equines (horses)) that were brought in for tertiary care at North Carolina State University Veterinary Hospital. C. difficile strains were characterized by toxin gene profiling, fluorescent PCR ribotyping, and antimicrobial susceptibility testing. 16S rRNA gene sequencing was done on animal feces to investigate the relationship between the presence of C. difficile and the gut microbiota in different hosts. Here, we show that C. difficile was recovered from 20.9% of samples (42/201), which included 33 canines, 2 felines, and 7 equines. Over 69% (29/42) of the isolates were toxigenic and belonged to 14 different ribotypes including ones known to cause CDI in humans. The presence of C. difficile results in a shift in the fecal microbial community structure in both canines and equines. Commensal Clostridium hiranonis was negatively associated with C. difficile in canines. Further experimentation showed a clear antagonistic relationship between the two strains in vitro, suggesting that commensal Clostridia might play a role in colonization resistance against C. difficile in different hosts.
Identifiants
pubmed: 33022384
pii: S1075-9964(20)30135-9
doi: 10.1016/j.anaerobe.2020.102279
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Bacterial Toxins
0
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
102279Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM119438
Pays : United States
Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest CMT is a scientific advisor to Locus Biosciences, a company engaged in the development of antimicrobial technologies. CMT is a consultant for Vedanta Biosciences.