Tabanids as possible pathogen vectors in Senegal (West Africa).
Leishmania donovani
MALDI-TOF MS
PCR
Senegal
Tabanids
Trypanosoma spp.
Journal
Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774
Informations de publication
Date de publication:
01 Oct 2020
01 Oct 2020
Historique:
received:
03
02
2020
accepted:
23
09
2020
entrez:
2
10
2020
pubmed:
3
10
2020
medline:
7
5
2021
Statut:
epublish
Résumé
Species of the Tabanidae are potent vectors of human and animal diseases, but they have not been thoroughly investigated to date. In Senegal (West Africa), little information is available on these dipterans. Our objective in this study was to investigate Senegalese tabanids and their diversity by using molecular and proteomics approaches, as well as their associated pathogens. A total of 171 female tabanids were collected, including 143 from Casamance and 28 from Niokolo-Koba. The samples were identified morphologically by PCR sequencing and by MALDI-TOF MS, and PCR analysis was employed for pathogen detection and blood-meal characterization. The morphological identification revealed four species concordantly with the molecular identification: Atylotus fuscipes (79.5%), Tabanus guineensis (16.4%), Chrysops distinctipennis (3.5%) and Tabanus taeniola (0.6%) (not identified by PCR). The molecular investigation of pathogens revealed the presence of Trypanosoma theileri (6.6%), Leishmania donovani (6.6%), Setaria digitata (1.5%), Rickettsia spp. (5.1%) and Anaplasmataceae bacteria (0.7%) in A. fuscipes. Tabanus guineensis was positive for L. donovani (35.7%), S. digitata (3.6%) and Anaplasmataceae (17.8%). Leishmania donovani has been detected in 50% of C. distinctipennis specimens and the only T. taeniola specimen. No Piroplasmida, Mansonella spp. or Coxeilla burnetii DNA was detected. In addition to humans (96.43%), Chlorocebus sabeus, a non-human primate, has been identified as a host of (3.57%) analysed tabanids. MALDI-TOF MS enabled us to correctly identify all tabanid species that had good quality spectra and to create a database for future identification. Tabanids in Senegal could be vectors of several pathogens threatening animal and public health. To fully characterize these dipterans, it is therefore necessary that researchers in entomology and infectiology employ molecular characterization and mass spectrometric techniques such as MALDI-TOF MS to analyse these dipterans in Senegal and West Africa.
Sections du résumé
BACKGROUND
BACKGROUND
Species of the Tabanidae are potent vectors of human and animal diseases, but they have not been thoroughly investigated to date. In Senegal (West Africa), little information is available on these dipterans. Our objective in this study was to investigate Senegalese tabanids and their diversity by using molecular and proteomics approaches, as well as their associated pathogens.
METHODS
METHODS
A total of 171 female tabanids were collected, including 143 from Casamance and 28 from Niokolo-Koba. The samples were identified morphologically by PCR sequencing and by MALDI-TOF MS, and PCR analysis was employed for pathogen detection and blood-meal characterization.
RESULTS
RESULTS
The morphological identification revealed four species concordantly with the molecular identification: Atylotus fuscipes (79.5%), Tabanus guineensis (16.4%), Chrysops distinctipennis (3.5%) and Tabanus taeniola (0.6%) (not identified by PCR). The molecular investigation of pathogens revealed the presence of Trypanosoma theileri (6.6%), Leishmania donovani (6.6%), Setaria digitata (1.5%), Rickettsia spp. (5.1%) and Anaplasmataceae bacteria (0.7%) in A. fuscipes. Tabanus guineensis was positive for L. donovani (35.7%), S. digitata (3.6%) and Anaplasmataceae (17.8%). Leishmania donovani has been detected in 50% of C. distinctipennis specimens and the only T. taeniola specimen. No Piroplasmida, Mansonella spp. or Coxeilla burnetii DNA was detected. In addition to humans (96.43%), Chlorocebus sabeus, a non-human primate, has been identified as a host of (3.57%) analysed tabanids. MALDI-TOF MS enabled us to correctly identify all tabanid species that had good quality spectra and to create a database for future identification.
CONCLUSIONS
CONCLUSIONS
Tabanids in Senegal could be vectors of several pathogens threatening animal and public health. To fully characterize these dipterans, it is therefore necessary that researchers in entomology and infectiology employ molecular characterization and mass spectrometric techniques such as MALDI-TOF MS to analyse these dipterans in Senegal and West Africa.
Identifiants
pubmed: 33004069
doi: 10.1186/s13071-020-04375-w
pii: 10.1186/s13071-020-04375-w
pmc: PMC7528383
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
500Subventions
Organisme : Agence Nationale de la Recherche
ID : ANR-10-IAHU-03
Références
Rev Elev Med Vet Pays Trop. 1968;21(3):365-73
pubmed: 5744936
Parasit Vectors. 2020 Jun 22;13(1):319
pubmed: 32571427
PLoS Negl Trop Dis. 2010 Apr 06;4(4):e654
pubmed: 20386603
Microorganisms. 2020 Mar 02;8(3):
pubmed: 32131458
Ann Parasitol Hum Comp. 1956 Jul-Sep;31(4):436-48
pubmed: 13363127
Infect Genet Evol. 2014 Dec;28:596-615
pubmed: 24727644
PLoS One. 2011 Mar 21;6(3):e14773
pubmed: 21445295
Parasitol Today. 1989 Mar;5(3):88-96
pubmed: 15463186
Proteomics. 2016 Dec;16(24):3148-3160
pubmed: 27862981
Parasit Vectors. 2019 Oct 22;12(1):495
pubmed: 31640746
PLoS Negl Trop Dis. 2010 Sep 14;4(9):
pubmed: 20856858
Emerg Infect Dis. 2011 May;17(5):883-5
pubmed: 21529402
Acta Trop. 2019 Sep;197:105069
pubmed: 31233728
Vet Parasitol. 2008 Jul 4;154(3-4):354-9
pubmed: 18495345
Emerg Infect Dis. 2004 May;10(5):810-7
pubmed: 15200813
Parasitology. 2017 Aug;144(9):1162-1178
pubmed: 28502276
Am J Trop Med Hyg. 2015 Sep;93(3):601-6
pubmed: 26078318
Methods Mol Biol. 2012;858:3-8
pubmed: 22684949
Vet Parasitol Reg Stud Reports. 2019 Dec;18:100332
pubmed: 31796173
Ann Trop Med Parasitol. 1989 Apr;83(2):167-72
pubmed: 2604456
Appl Microbiol Biotechnol. 2014 Apr;98(8):3425-36
pubmed: 24522727
J Med Entomol. 2018 Jan 10;55(1):112-121
pubmed: 29040652
Parasit Vectors. 2011 Mar 04;4:31
pubmed: 21375725
Methods Mol Biol. 2012;858:17-46
pubmed: 22684951
Proc Natl Acad Sci U S A. 1989 Aug;86(16):6196-200
pubmed: 2762322
Parasitol Res. 1987;73(5):421-4
pubmed: 3658973
Eur J Clin Microbiol Infect Dis. 2011 Feb;30(2):209-18
pubmed: 20936316
Bioessays. 2011 Feb;33(2):135-47
pubmed: 21184470
Microbes Infect. 2010 Dec;12(14-15):1219-25
pubmed: 20868766
Parasite. 2009 Sep;16(3):231-3
pubmed: 19839270
Bull Soc Pathol Exot Filiales. 1958 Nov-Dec;51(6):992-8
pubmed: 13662810
PLoS One. 2012;7(1):e28266
pubmed: 22276095
Zookeys. 2018 Jun 26;(769):117-144
pubmed: 29988760
Exp Parasitol. 2003 Nov-Dec;105(3-4):226-31
pubmed: 14990316
Med Vet Entomol. 2010 Dec;24(4):382-410
pubmed: 20649754
Parasitol Res. 2005 Feb;95(3):186-92
pubmed: 15619129
Comp Immunol Microbiol Infect Dis. 2012 Mar;35(2):145-50
pubmed: 22277830
Ticks Tick Borne Dis. 2015 Mar;6(2):198-203
pubmed: 25583345
Vet Parasitol. 2018 Aug 15;259:35-43
pubmed: 30056981
Parasite Epidemiol Control. 2016 Apr 16;1(2):85-89
pubmed: 29988182
Rev Elev Med Vet Pays Trop. 1983;36(4):351-3
pubmed: 6675072
PLoS Negl Trop Dis. 2017 Jul 24;11(7):e0005762
pubmed: 28742123
Acta Trop. 2015 Oct;150:52-8
pubmed: 26126785
Comp Immunol Microbiol Infect Dis. 2020 Apr;69:101412
pubmed: 31981798
J Clin Microbiol. 2004 Nov;42(11):5249-55
pubmed: 15528722
Mol Mar Biol Biotechnol. 1994 Oct;3(5):294-9
pubmed: 7881515
Med Vet Entomol. 2017 Dec;31(4):438-448
pubmed: 28722283
PLoS One. 2013 Aug 15;8(8):e72380
pubmed: 23977292
Parasite. 2013;20:26
pubmed: 23985165
J Parasitol Res. 2019 Sep 2;2019:9282690
pubmed: 31565426
J Med Entomol. 1976 Dec 8;13(3):225-75
pubmed: 137982