How Relevant Are Bone Marrow-Derived Mast Cells (BMMCs) as Models for Tissue Mast Cells? A Comparative Transcriptome Analysis of BMMCs and Peritoneal Mast Cells.
ADAMTS9 Protein
/ genetics
Animals
B-Lymphocytes
/ cytology
Biomarkers
/ metabolism
Bone Marrow Cells
/ cytology
Complement C2
/ genetics
Female
Gene Expression Regulation
Glial Cell Line-Derived Neurotrophic Factor Receptors
/ genetics
Histidine Decarboxylase
/ genetics
Homeodomain Proteins
/ genetics
Macrophages
/ cytology
Mast Cells
/ cytology
Mice
Mice, Inbred BALB C
Organ Specificity
Peritoneum
/ cytology
Proto-Oncogene Proteins c-myb
/ genetics
Receptors, G-Protein-Coupled
/ genetics
Receptors, Immunologic
/ genetics
Receptors, Interleukin-3
/ genetics
Serine Endopeptidases
/ genetics
Transcriptome
FcεRI
chymase
heparin
histamine
in vitro model
mRNA
mast cell
serine protease
transcriptome
tryptase
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
17 09 2020
17 09 2020
Historique:
received:
29
07
2020
revised:
14
09
2020
accepted:
16
09
2020
entrez:
22
9
2020
pubmed:
23
9
2020
medline:
25
3
2021
Statut:
epublish
Résumé
Bone marrow-derived mast cells (BMMCs) are often used as a model system for studies of the role of MCs in health and disease. These cells are relatively easy to obtain from total bone marrow cells by culturing under the influence of IL-3 or stem cell factor (SCF). After 3 to 4 weeks in culture, a nearly homogenous cell population of toluidine blue-positive cells are often obtained. However, the question is how relevant equivalents these cells are to normal tissue MCs. By comparing the total transcriptome of purified peritoneal MCs with BMMCs, here we obtained a comparative view of these cells. We found several important transcripts that were expressed at very high levels in peritoneal MCs, but were almost totally absent from the BMMCs, including the major chymotryptic granule protease Mcpt4, the neurotrophin receptor Gfra2, the substance P receptor Mrgprb2, the metalloprotease Adamts9 and the complement factor 2 (C2). In addition, there were a number of other molecules that were expressed at much higher levels in peritoneal MCs than in BMMCs, including the transcription factors Myb and Meis2, the MilR1 (Allergin), Hdc (Histidine decarboxylase), Tarm1 and the IL-3 receptor alpha chain. We also found many transcripts that were highly expressed in BMMCs but were absent or expressed at low levels in the peritoneal MCs. However, there were also numerous MC-related transcripts that were expressed at similar levels in the two populations of cells, but almost absent in peritoneal macrophages and B cells. These results reveal that the transcriptome of BMMCs shows many similarities, but also many differences to that of tissue MCs. BMMCs can thereby serve as suitable models in many settings concerning the biology of MCs, but our findings also emphasize that great care should be taken when extrapolating findings from BMMCs to the in vivo function of tissue-resident MCs.
Identifiants
pubmed: 32957735
pii: cells9092118
doi: 10.3390/cells9092118
pmc: PMC7564378
pii:
doi:
Substances chimiques
Biomarkers
0
Complement C2
0
Gfra2 protein, mouse
0
Glial Cell Line-Derived Neurotrophic Factor Receptors
0
Homeodomain Proteins
0
Milr1 protein, mouse
0
Mrg1 protein, mouse
0
Mrgprb2 protein, mouse
0
Proto-Oncogene Proteins c-myb
0
Receptors, G-Protein-Coupled
0
Receptors, Immunologic
0
Receptors, Interleukin-3
0
TARM1 protein, mouse
0
Serine Endopeptidases
EC 3.4.21.-
mast cell protease 4
EC 3.4.21.-
ADAMTS9 Protein
EC 3.4.24.-
Adamts9 protein, mouse
EC 3.4.24.-
Histidine Decarboxylase
EC 4.1.1.22
Types de publication
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Références
Scand J Immunol. 1993 Feb;37(2):203-8
pubmed: 8434231
J Biol Chem. 2001 Feb 9;276(6):3772-7
pubmed: 11035040
PLoS One. 2016 Jun 23;11(6):e0158104
pubmed: 27337047
Eur J Immunol. 1998 Nov;28(11):3730-7
pubmed: 9842915
Development. 2015 Jul 1;142(13):2230-6
pubmed: 26130754
Proc Natl Acad Sci U S A. 1986 Sep;83(17):6485-8
pubmed: 3462707
Front Immunol. 2020 Apr 08;11:564
pubmed: 32322252
Biol Chem. 2014 Jan;395(1):15-49
pubmed: 23969467
Exp Dermatol. 2017 Mar;26(3):255-262
pubmed: 27619074
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2559-61
pubmed: 6166010
Int Immunol. 2000 Apr;12(4):573-80
pubmed: 10744659
J Struct Biol. 2002 Mar;137(3):313-21
pubmed: 12096899
Allergol Int. 2016 Jan;65(1):3-15
pubmed: 26666482
Blood. 2010 Jun 17;115(24):4981-90
pubmed: 20233968
Eur J Immunol. 2000 Dec;30(12):3396-402
pubmed: 11093157
J Exp Med. 1997 Jan 6;185(1):13-29
pubmed: 8996238
Leuk Lymphoma. 1992 Jun;7(3):243-50
pubmed: 1477652
J Immunol. 1987 Apr 15;138(8):2611-5
pubmed: 3549903
PLoS One. 2012;7(10):e48308
pubmed: 23118978
Allergy. 2006 Sep;61(9):1063-70
pubmed: 16918508
DNA Cell Biol. 1998 Jan;17(1):27-37
pubmed: 9468220
Scand J Immunol. 1996 Jul;44(1):54-61
pubmed: 8693292
Eur J Immunol. 1998 Mar;28(3):1022-33
pubmed: 9541598
Adv Exp Med Biol. 2011;716:212-34
pubmed: 21713659
Cells. 2020 Jan 14;9(1):
pubmed: 31947690
Scand J Immunol. 1994 May;39(5):489-98
pubmed: 8191224
Semin Immunopathol. 2016 Sep;38(5):539-48
pubmed: 27126100
Histochem Cell Biol. 2002 Jul;118(1):41-9
pubmed: 12122446
Br J Haematol. 1989 Nov;73(3):348-55
pubmed: 2513867
Cell Tissue Bank. 2015 Dec;16(4):487-95
pubmed: 25605061
Nature. 1981 May 28;291(5813):332-4
pubmed: 6972009
J Immunol. 2007 May 15;178(10):6465-75
pubmed: 17475876
Proc Natl Acad Sci U S A. 2010 Feb 9;107(6):2568-73
pubmed: 20133793
Blood. 2008 Oct 1;112(7):2969-72
pubmed: 18635811
J Histochem Cytochem. 2012 Dec;60(12):950-62
pubmed: 22899859
Int J Mol Sci. 2019 Sep 17;20(18):
pubmed: 31533351
J Leukoc Biol. 1985 Aug;38(2):213-30
pubmed: 2993459
FEBS Lett. 2001 Jul 27;502(1-2):53-6
pubmed: 11478947
PLoS One. 2014 May 09;9(5):e96903
pubmed: 24816777
Eur J Immunol. 1992 Aug;22(8):2025-32
pubmed: 1639103
J Immunol. 2017 Feb 15;198(4):1474-1483
pubmed: 28053237
Mol Cell Biol. 2004 Jul;24(13):6067-75
pubmed: 15199160
Nature. 1999 Aug 19;400(6746):773-6
pubmed: 10466727
Nature. 1989 May 4;339(6219):64-7
pubmed: 2469965
J Clin Invest. 2011 Oct;121(10):4180-91
pubmed: 21926462
Leukemia. 1994 Sep;8(9):1579-84
pubmed: 8090034
Immunol Rev. 2018 Mar;282(1):35-46
pubmed: 29431204
Nature. 1988 Dec 15;336(6200):684-7
pubmed: 3143916
Gene. 1990 Sep 14;93(2):235-40
pubmed: 2121613
Blood. 1999 May 15;93(10):3473-86
pubmed: 10233900
Int J Mol Sci. 2019 Nov 06;20(22):
pubmed: 31698677
J Leukoc Biol. 1985 Apr;37(4):407-22
pubmed: 3855947
Proc Natl Acad Sci U S A. 1990 Apr;87(8):3230-4
pubmed: 2326280
Blood. 2018 May 24;131(21):e1-e11
pubmed: 29588278
Exp Dermatol. 2017 May;26(5):446-449
pubmed: 27677092
FEBS Lett. 2003 Apr 10;540(1-3):111-6
pubmed: 12681493
Front Immunol. 2017 Dec 19;8:1749
pubmed: 29312297
J Immunol. 1976 May;116(5):1490-5
pubmed: 178799
Biochem Biophys Res Commun. 2014 Aug 22;451(2):314-8
pubmed: 25094046
Immunol Rev. 2018 Mar;282(1):121-150
pubmed: 29431212
Immunol Rev. 2009 Nov;232(1):195-217
pubmed: 19909365
PLoS One. 2015 Nov 16;10(11):e0143091
pubmed: 26569620
Int J Mol Sci. 2019 Dec 29;21(1):
pubmed: 31905768
Blood. 2014 Apr 24;123(17):e58-67
pubmed: 24671954
Nat Immunol. 2016 Jul;17(7):878-87
pubmed: 27135604
Exp Dermatol. 2016 Jun;25(6):434-9
pubmed: 26706922
Biochem J. 1989 Oct 1;263(1):105-13
pubmed: 2532501
Eur J Immunol. 2000 Sep;30(9):2660-8
pubmed: 11009100
Am J Pathol. 2012 Sep;181(3):875-86
pubmed: 22901752
Exp Dermatol. 2010 Sep;19(9):845-7
pubmed: 20545757
Blood. 2015 Jan 8;125(2):358-69
pubmed: 25398936
FASEB J. 2001 Dec;15(14):2763-5
pubmed: 11687508
Eur J Immunol. 1990 Sep;20(9):1941-7
pubmed: 2209700
Adv Immunol. 1994;55:1-96
pubmed: 7508174
Eur J Immunol. 1991 Jul;21(7):1611-21
pubmed: 2060576
Int J Mol Sci. 2019 Oct 17;20(20):
pubmed: 31627390
J Exp Med. 1999 Nov 15;190(10):1527-34
pubmed: 10562326
Blood. 1985 Aug;66(2):312-8
pubmed: 2410064
Science. 2006 Jul 28;313(5786):526-30
pubmed: 16873664