Reduced phosphorus intake throughout gestation and lactation of sows is mitigated by transcriptional adaptations in kidney and intestine.
Dietary phosphorus
Gestational diets
Lactating sows
Mineral requirement
Monogastric farm animals
Phosphorus homeostasis
Journal
BMC genomics
ISSN: 1471-2164
Titre abrégé: BMC Genomics
Pays: England
ID NLM: 100965258
Informations de publication
Date de publication:
11 Sep 2020
11 Sep 2020
Historique:
received:
05
06
2020
accepted:
03
09
2020
entrez:
12
9
2020
pubmed:
13
9
2020
medline:
13
4
2021
Statut:
epublish
Résumé
The environmental impact of pig farming need to be reduced, with phosphorus (P) being of particular interest. Specified dietary regimens and management systems contribute to meet environmental concerns and reduce economic constrains. However, pregnant and lactating sows represent vulnerable individuals, whose reproductive potential and metabolic health status relies on adequate supply of macro- and micronutrients. The aim of this study was to investigate, whether sows fed with a dietary P content that is below or above current recommendations are capable to maintain mineral homeostasis during the reproduction cycle and which endogenous mechanisms are retrieved therefore in kidney and jejunum. Nulliparous gilts were fed iso-energetic diets with recommended (M), reduced (L), or high (H) amounts of mineral P supplements throughout gestation and lactation periods. Blood metabolites and hormones referring to the P homeostasis were retrieved prior to term (110 days of gestation) and at weaning (28 days of lactation). Transcriptional responses in kidney cortex and jejunal mucosa were analyzed using RNA sequencing. The variable dietary P content neither led to an aberration on fertility traits such as total weaned piglets nor to an effect on the weight pattern throughout gestation and lactation. Serum parameters revealed a maintained P homeostasis as reflected by unaltered inorganic P and calcium levels in L and H fed groups. The serum calcitriol levels were increased in lactating L sows. The endocrine responses to the dietary challenge were reflected at the transcriptional level. L diets led to an increase in CYP27B1 expression in the kidney compared to the H group and to an altered gene expression associated with lipid metabolism in the kidney and immune response in the jejunum. Our results suggest that current P requirements for gestating and lactating sows are sufficient and over supplementation of mineral P is not required. Shifts in renal and jejunal expression patterns between L and H groups indicate an affected intermediate metabolism, which long-term relevance needs to be further clarified.
Sections du résumé
BACKGROUND
BACKGROUND
The environmental impact of pig farming need to be reduced, with phosphorus (P) being of particular interest. Specified dietary regimens and management systems contribute to meet environmental concerns and reduce economic constrains. However, pregnant and lactating sows represent vulnerable individuals, whose reproductive potential and metabolic health status relies on adequate supply of macro- and micronutrients. The aim of this study was to investigate, whether sows fed with a dietary P content that is below or above current recommendations are capable to maintain mineral homeostasis during the reproduction cycle and which endogenous mechanisms are retrieved therefore in kidney and jejunum. Nulliparous gilts were fed iso-energetic diets with recommended (M), reduced (L), or high (H) amounts of mineral P supplements throughout gestation and lactation periods. Blood metabolites and hormones referring to the P homeostasis were retrieved prior to term (110 days of gestation) and at weaning (28 days of lactation). Transcriptional responses in kidney cortex and jejunal mucosa were analyzed using RNA sequencing.
RESULTS
RESULTS
The variable dietary P content neither led to an aberration on fertility traits such as total weaned piglets nor to an effect on the weight pattern throughout gestation and lactation. Serum parameters revealed a maintained P homeostasis as reflected by unaltered inorganic P and calcium levels in L and H fed groups. The serum calcitriol levels were increased in lactating L sows. The endocrine responses to the dietary challenge were reflected at the transcriptional level. L diets led to an increase in CYP27B1 expression in the kidney compared to the H group and to an altered gene expression associated with lipid metabolism in the kidney and immune response in the jejunum.
CONCLUSIONS
CONCLUSIONS
Our results suggest that current P requirements for gestating and lactating sows are sufficient and over supplementation of mineral P is not required. Shifts in renal and jejunal expression patterns between L and H groups indicate an affected intermediate metabolism, which long-term relevance needs to be further clarified.
Identifiants
pubmed: 32917128
doi: 10.1186/s12864-020-07049-0
pii: 10.1186/s12864-020-07049-0
pmc: PMC7488499
doi:
Substances chimiques
Phosphorus, Dietary
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
626Subventions
Organisme : ERA-NET SusAn
ID : 2817ERA02D
Références
J Nutr Biochem. 2006 Jun;17(6):385-95
pubmed: 16311027
Bone. 2004 Apr;34(4):629-37
pubmed: 15050893
Annu Rev Biochem. 2005;74:739-89
pubmed: 15952902
Front Immunol. 2018 Apr 18;9:813
pubmed: 29720980
Cold Spring Harb Perspect Biol. 2011 Jul 01;3(7):
pubmed: 21576254
J Anim Sci. 2009 Apr;87(4):1518-27
pubmed: 19028841
J Environ Sci Health B. 2017 Jun 3;52(6):395-401
pubmed: 28277077
Am J Physiol Gastrointest Liver Physiol. 2017 Apr 1;312(4):G355-G366
pubmed: 28232455
Cold Spring Harb Perspect Biol. 2011 Dec 01;3(12):
pubmed: 21900404
J Clin Biochem Nutr. 2012 May;50(3):227-33
pubmed: 22573926
Bioinformatics. 2015 Jan 15;31(2):166-9
pubmed: 25260700
Nat Protoc. 2016 Sep;11(9):1650-67
pubmed: 27560171
J Anim Physiol Anim Nutr (Berl). 2020 Mar 17;:
pubmed: 32180287
Nutrients. 2019 Feb 20;11(2):
pubmed: 30791512
Eur J Biochem. 1997 Feb 15;244(1):1-14
pubmed: 9063439
In Vivo. 2017 01-02;31(1):145-150
pubmed: 28064234
BMC Genomics. 2018 Mar 20;19(1):207
pubmed: 29554878
J Anim Sci Biotechnol. 2019 May 28;10:47
pubmed: 31149337
Orthop Clin North Am. 1984 Oct;15(4):597-612
pubmed: 6387574
J Anim Sci. 2006 Sep;84(9):2316-37
pubmed: 16908634
Genome Biol. 2014;15(12):550
pubmed: 25516281
Physiology (Bethesda). 2009 Feb;24:17-25
pubmed: 19196648
Nucleic Acids Res. 2011 Jul;39(Web Server issue):W316-22
pubmed: 21715386
J Anim Sci. 2008 Oct;86(10):2585-95
pubmed: 18502882
Cell Stress Chaperones. 2017 Mar;22(2):191-200
pubmed: 27966060
J Biol Chem. 2010 Mar 26;285(13):9420-8
pubmed: 20124409
Nutr Res Rev. 2015 Jun;28(1):67-82
pubmed: 26004147
Reproduction. 2005 Jun;129(6):757-63
pubmed: 15923391
J Anim Sci. 1997 Aug;75(8):2129-38
pubmed: 9263060
Physiol Rev. 2016 Apr;96(2):449-547
pubmed: 26887676
Endocrinology. 2005 Dec;146(12):5358-64
pubmed: 16123154
J Bone Miner Res. 2007 Apr;22(4):617-27
pubmed: 17227223
PLoS Comput Biol. 2017 Nov 3;13(11):e1005752
pubmed: 29099853
Curr Opin Lipidol. 2004 Jun;15(3):261-7
pubmed: 15166781
Int J Mol Sci. 2016 Nov 30;17(12):
pubmed: 27916883
Am J Physiol Endocrinol Metab. 2016 Apr 1;310(7):E526-38
pubmed: 26786774
Cells Tissues Organs. 2011;194(2-4):205-10
pubmed: 21597266
J Bone Miner Res. 2013 Jan;28(1):46-55
pubmed: 22886720
J Anim Sci. 2007 Oct;85(10):2498-507
pubmed: 17565069
Endocrinology. 2013 Feb;154(2):609-22
pubmed: 23307792
J Anim Sci. 1990 Feb;68(2):573-82
pubmed: 2179195
Am J Physiol Renal Physiol. 2003 Dec;285(6):F1233-43
pubmed: 12952858
PLoS One. 2012;7(5):e37787
pubmed: 22662222
N Engl J Med. 2011 Feb 3;364(5):432-42
pubmed: 21288095
Br J Nutr. 2010 Feb;103(4):561-8
pubmed: 19781123
Nat Commun. 2019 Oct 16;10(1):4693
pubmed: 31619668
Biochem Biophys Res Commun. 2003 Oct 10;310(1):128-34
pubmed: 14511659
J Anim Sci. 2009 Apr;87(14 Suppl):E123-32
pubmed: 19098235
Int J Mol Sci. 2019 Nov 08;20(22):
pubmed: 31717287
J Bone Miner Res. 1997 Feb;12(2):165-71
pubmed: 9041047
PLoS One. 2012;7(1):e29483
pubmed: 22235299
PLoS One. 2016 May 17;11(5):e0155386
pubmed: 27187182
Ann N Y Acad Sci. 1980;355:1-17
pubmed: 7015957
J Biol Chem. 2003 Sep 5;278(36):33920-7
pubmed: 12819193
Front Genet. 2019 Jun 28;10:590
pubmed: 31316547
Obesity (Silver Spring). 2014 Sep;22(9):2010-7
pubmed: 24845055