Peculiarities of Cell Seeding on Electroformed Polycaprolactone Scaffolds Modified with Surface-Active Agents Triton X-100 and Polyvinylpyrrolidone.
Biocompatible Materials
Cell Proliferation
/ drug effects
Electrochemical Techniques
Fibroblasts
/ cytology
Humans
Hydrophobic and Hydrophilic Interactions
Octoxynol
/ chemistry
Polyesters
/ chemistry
Porosity
Povidone
/ chemistry
Primary Cell Culture
Skin
/ cytology
Surface-Active Agents
/ chemistry
Tissue Engineering
Tissue Scaffolds
Triton Х-100
cell culture
polyvinylpyrrolidone
synthetic scaffold
tissue engineering
Journal
Bulletin of experimental biology and medicine
ISSN: 1573-8221
Titre abrégé: Bull Exp Biol Med
Pays: United States
ID NLM: 0372557
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
17
02
2020
pubmed:
11
9
2020
medline:
10
7
2021
entrez:
10
9
2020
Statut:
ppublish
Résumé
We compared the capability of human fibroblasts to populate porous polycaprolactone (PCL) scaffolds modified during fabrication with surface-active agents Triton Х-100 (type 1 scaffold) and polyvinylpyrrolidone (type 2 scaffold). The mean fiber diameter in both scaffolds was almost the same: 3.90±2.19 and 2.46±2.15 μ, respectively. Type 1 scaffold had higher surface density and hydrophilicity, when type 2 scaffold was 1.6 times thicker. The cells were seeded on the scaffolds by the dynamic seeding technique and then cultured in Petri dishes with nutrient medium in a humid atmosphere. During 3-day culturing, no cell release from the matrix was noted. DAPI staining proved the presence of cells in both scaffolds. However, in type 1 scaffold the cells populated the whole thickness, while in type 2 scaffold, the cells were present only in the superficial layer. These findings suggest that PCL scaffolds modified with Triton Х-100 or polyvinylpyrrolidone are not cytotoxic, but the structure of the scaffold treated with Triton Х-100 is more favorable for population with cells.
Identifiants
pubmed: 32910398
doi: 10.1007/s10517-020-04936-0
pii: 10.1007/s10517-020-04936-0
doi:
Substances chimiques
Biocompatible Materials
0
Polyesters
0
Surface-Active Agents
0
polycaprolactone
24980-41-4
Octoxynol
9002-93-1
Povidone
FZ989GH94E
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM