Effect of grape seed extract on skin fibroblasts exposed to UVA light and its photostability in sunscreen formulation.
UVA
anti-aging
fibroblasts
grape seed extract
reflection and transmission UV spectrophotometer
sunscreen
Journal
Journal of cosmetic dermatology
ISSN: 1473-2165
Titre abrégé: J Cosmet Dermatol
Pays: England
ID NLM: 101130964
Informations de publication
Date de publication:
Apr 2021
Apr 2021
Historique:
revised:
12
08
2020
received:
08
05
2020
accepted:
24
08
2020
pubmed:
7
9
2020
medline:
15
5
2021
entrez:
6
9
2020
Statut:
ppublish
Résumé
Grape seed extract (GSE) is rich in polyphenolic compounds, particularly (+)-catechin (C) and (-)-epicatechin (EC). Strong antioxidant activity of these compounds makes GSE to be value-added to the cosmetics with anti-aging properties. However, a lack of stability in different environmental conditions makes GSE challenging for the development of photostable cosmetic sunscreen products. To evaluate photoprotective effects of GSE on human dermal fibroblasts irradiated with UVA light and assess photostability of catechins in cream formulations containing GSE alone or in combination with octyl methoxycinnamate (OMC). MTT assay was used to assess protective effects of GSE on fibroblasts irradiated with UVA light. A photostability of C and EC in GSE and in cream formulation containing GSE was investigated using high-performance liquid chromatography and confirmed by reflection and transmission spectrophotometry using Transpore™ tapes and polymethacrylate (PMMA) plates as substrates. High UVA doses damaged fibroblast structure and inhibited their growth. However, GSE increased cell viability and effectively protected them from UVA damage. Photostability of C and EC was achieved by combination of GSE and OMC that also improved absorption capacity of UV filter and increased overall efficacy of formulation. PMMA plates showed better applicability for in vitro photostability testing of sunscreen formulations. However, despite the instability of Transpore® tape under heat from UV exposure, it can still be economically a substrate of alternative choice for screening. GSE can be used as an effective and sustainable natural resource for prevention of UV-induced skin damage providing long-term protection against premature skin aging.
Sections du résumé
BACKGROUND
BACKGROUND
Grape seed extract (GSE) is rich in polyphenolic compounds, particularly (+)-catechin (C) and (-)-epicatechin (EC). Strong antioxidant activity of these compounds makes GSE to be value-added to the cosmetics with anti-aging properties. However, a lack of stability in different environmental conditions makes GSE challenging for the development of photostable cosmetic sunscreen products.
AIMS
OBJECTIVE
To evaluate photoprotective effects of GSE on human dermal fibroblasts irradiated with UVA light and assess photostability of catechins in cream formulations containing GSE alone or in combination with octyl methoxycinnamate (OMC).
METHODS
METHODS
MTT assay was used to assess protective effects of GSE on fibroblasts irradiated with UVA light. A photostability of C and EC in GSE and in cream formulation containing GSE was investigated using high-performance liquid chromatography and confirmed by reflection and transmission spectrophotometry using Transpore™ tapes and polymethacrylate (PMMA) plates as substrates.
RESULTS
RESULTS
High UVA doses damaged fibroblast structure and inhibited their growth. However, GSE increased cell viability and effectively protected them from UVA damage. Photostability of C and EC was achieved by combination of GSE and OMC that also improved absorption capacity of UV filter and increased overall efficacy of formulation. PMMA plates showed better applicability for in vitro photostability testing of sunscreen formulations. However, despite the instability of Transpore® tape under heat from UV exposure, it can still be economically a substrate of alternative choice for screening.
CONCLUSIONS
CONCLUSIONS
GSE can be used as an effective and sustainable natural resource for prevention of UV-induced skin damage providing long-term protection against premature skin aging.
Substances chimiques
Grape Seed Extract
0
Sunscreening Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1271-1282Subventions
Organisme : Faculty of Pharmaceutical Sciences, Khon Kaen University
Informations de copyright
© 2020 Wiley Periodicals LLC.
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