Using Matrix-Assisted Laser Desorption/Ionization Time of Flight Spectra To Elucidate Species Boundaries by Matching to Translated DNA Databases.
Aeromonas
/ chemistry
Bacteria
/ genetics
Bacterial Proteins
/ chemistry
Bacteriological Techniques
/ methods
Databases, Nucleic Acid
Fresh Water
/ microbiology
Genome, Bacterial
/ genetics
Massachusetts
Reproducibility of Results
Software
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
/ methods
Vibrio
/ chemistry
MALDI
bacteria
identification
matrix-assisted laser desorption/ionization time of flight mass spectrometry
ribosomal
species
Journal
Journal of the American Society for Mass Spectrometry
ISSN: 1879-1123
Titre abrégé: J Am Soc Mass Spectrom
Pays: United States
ID NLM: 9010412
Informations de publication
Date de publication:
02 Jan 2020
02 Jan 2020
Historique:
entrez:
4
9
2020
pubmed:
4
9
2020
medline:
17
7
2021
Statut:
ppublish
Résumé
A method has been established to map a bacterial colony to the ever-expanding database of publicly available bacterial genomes by means of matrix-assisted laser desorption/ionization (MALDI) spectra. To accomplish this, spectra are mapped to the predicted masses of ∼65 families of mostly ribosomal proteins. Each of the ∼40 000 bacterial strains in the database receives scores, together with tables listing identified protein sequences and how the highest ranking strains are related to one another. The approach was first confirmed with 16 distinct species of bacteria from the
Identifiants
pubmed: 32881510
doi: 10.1021/jasms.9b00031
doi:
Substances chimiques
Bacterial Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM