Association between genetic polymorphisms in the promoter region of the defensin beta 1 gene and persistent apical periodontitis.
DEFB1
apical periodontitis
genetic polymorphisms
innate immunity
Journal
International endodontic journal
ISSN: 1365-2591
Titre abrégé: Int Endod J
Pays: England
ID NLM: 8004996
Informations de publication
Date de publication:
Jan 2021
Jan 2021
Historique:
received:
22
04
2020
accepted:
26
08
2020
pubmed:
3
9
2020
medline:
30
12
2020
entrez:
3
9
2020
Statut:
ppublish
Résumé
To evaluate the association between the promoter region of defensin beta 1 (DEFB1) genetic polymorphisms and persistent apical periodontitis (PAP) in Brazilian patients. Seventy-three patients with post-treatment PAP (PAP group) and 89 patients with root filled teeth with healed and healthy periradicular tissues (healed group) were included (all teeth had apical periodontitis lesions at the beginning of the treatment). Patients who had undergone at least 1 year of follow-up after root canal treatment were recalled, and their genomic DNA was extracted from saliva. Two single nucleotide polymorphisms (SNPs) in DEFB1 at the g. -52G>A (rs1799946) and g. -20G>A (rs11362) positions were analysed using real-time polymerase chain reaction. The chi-squared test was performed, and the odds ratios were calculated using Epi Info 3.5.2. Logistic regression analysis in the codominant model, using the time of follow-up as a variable, was used to evaluate the SNP-SNP interaction. All tests were performed with an established alpha of 0.05 (P = 0.05). For the rs11362 polymorphism in the codominant and recessive models, patients who carried two copies of the T allele had a significantly lower risk of developing PAP (P = 0.040 and P = 0.031, respectively). For the rs1799946 polymorphism in DEFB1 in the codominant and recessive models, carrying one copy of the T allele significantly increased the risk of developing PAP (P = 0.007 and P = 0.031, respectively). In the logistic regression, both polymorphisms were associated with PAP as well as the SNP-SNP interaction (P < 0.0001). Polymorphisms in DEFB1 genes were associated with the development of post-treatment persistent apical periodontitis.
Substances chimiques
DEFB1 protein, human
0
beta-Defensins
0
Types de publication
Journal Article
Langues
eng
Pagination
38-45Informations de copyright
© 2020 International Endodontic Journal. Published by John Wiley & Sons Ltd.
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