SARS-CoV-2 Antibody Responses Correlate with Resolution of RNAemia But Are Short-Lived in Patients with Mild Illness.

Journal

medRxiv : the preprint server for health sciences
Titre abrégé: medRxiv
Pays: United States
ID NLM: 101767986

Informations de publication

Date de publication:
17 Aug 2020
Historique:
pubmed: 26 8 2020
medline: 26 8 2020
entrez: 26 8 2020
Statut: epublish

Résumé

SARS-CoV-2-specific antibodies, particularly those preventing viral spike receptor binding domain (RBD) interaction with host angiotensin-converting enzyme 2 (ACE2) receptor, could offer protective immunity, and may affect clinical outcomes of COVID-19 patients. We analyzed 625 serial plasma samples from 40 hospitalized COVID-19 patients and 170 SARS-CoV-2-infected outpatients and asymptomatic individuals. Severely ill patients developed significantly higher SARS-CoV-2-specific antibody responses than outpatients and asymptomatic individuals. The development of plasma antibodies was correlated with decreases in viral RNAemia, consistent with potential humoral immune clearance of virus. Using a novel competition ELISA, we detected antibodies blocking RBD-ACE2 interactions in 68% of inpatients and 40% of outpatients tested. Cross-reactive antibodies recognizing SARS-CoV RBD were found almost exclusively in hospitalized patients. Outpatient and asymptomatic individuals' serological responses to SARS-CoV-2 decreased within 2 months, suggesting that humoral protection may be short-lived.

Identifiants

DOI: 10.1101/2020.08.15.20175794 PMID: 32839786 PMC: PMC7444305
pubmed: 32839786
doi: 10.1101/2020.08.15.20175794
pmc: PMC7444305
pii:
doi:

Types de publication

Preprint

Langues

eng

Déclaration de conflit d'intérêts

Competing interests: The authors declare no competing interests.

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Auteurs

Katharina Röltgen (K)

Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.

Oliver F Wirz (OF)

Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.

Bryan A Stevens (BA)

Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.

Abigail E Powell (AE)

Stanford ChEM-H and Department of Biochemistry, Stanford University School of Medicine, Stanford, CA, USA.

Catherine A Hogan (CA)

Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.

Javaria Najeeb (J)

Department of Structural Biology, Stanford University, Stanford, USA.

Molly Hunter (M)

ATUM, Newark, CA, USA.

Malaya K Sahoo (MK)

Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.

ChunHong Huang (C)

Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.

Fumiko Yamamoto (F)

Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.

Justin Manalac (J)

Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.

Ana R Otrelo-Cardoso (AR)

Department of Structural Biology, Stanford University, Stanford, USA.

Tho D Pham (TD)

Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.
Stanford Blood Center, Palo Alto, CA, USA.

Arjun Rustagi (A)

Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University, Stanford, CA, USA.

Angela J Rogers (AJ)

Department of Medicine, Division of Pulmonary, Allergy and Critical Care Medicine, Stanford University, Stanford, CA, USA.

Nigam H Shah (NH)

Stanford Center for Biomedical Informatics Research, Stanford University, Stanford, California, USA.

Catherine A Blish (CA)

Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University, Stanford, CA, USA.
Chan Zuckerberg Biohub, San Francisco, CA, USA.

Jennifer R Cochran (JR)

Department of Bioengineering, Stanford University, Stanford, CA, USA.

Kari C Nadeau (KC)

Department of Medicine, Division of Pulmonary, Allergy and Critical Care Medicine, Stanford University, Stanford, CA, USA.
Sean N. Parker Center for Allergy and Asthma Research, Stanford, CA, USA.

Theodore S Jardetzky (TS)

Department of Structural Biology, Stanford University, Stanford, USA.

James L Zehnder (JL)

Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.

Taia T Wang (TT)

Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University, Stanford, CA, USA.
Chan Zuckerberg Biohub, San Francisco, CA, USA.
Department of Microbiology and Immunology, Stanford University, Stanford, CA, USA.

Peter S Kim (PS)

Stanford ChEM-H and Department of Biochemistry, Stanford University School of Medicine, Stanford, CA, USA.
Chan Zuckerberg Biohub, San Francisco, CA, USA.

Saurabh Gombar (S)

Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.

Robert Tibshirani (R)

Department of Biomedical Data Sciences, Stanford University, Stanford, CA, USA.
Department of Statistics, Stanford University, Stanford, CA, USA.

Benjamin A Pinsky (BA)

Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.
Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University, Stanford, CA, USA.

Scott D Boyd (SD)

Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.
Sean N. Parker Center for Allergy and Asthma Research, Stanford, CA, USA.

Classifications MeSH