High-Throughput Cell Death Assays with Single-Cell and Population-Level Analyses Using Real-Time Kinetic Labeling (SPARKL).

Cell Death Cytological Techniques Fluorescent Dyes Kinetics

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
19 06 2020
Historique:
entrez: 15 8 2020
pubmed: 15 8 2020
medline: 15 8 2020
Statut: ppublish

Résumé

High-throughput cytostatic and cell death assays are a critical component of pharmacological screens and mechanism-based interrogations into cellular biology. We developed a method for single-cell and population-level analyses using real-time kinetic labeling (abbreviated "SPARKL") with non-toxic fluorescent probes and high-content live-cell imagers. The protocols herein detail the steps, specifics, and suggested utilization of the SPARKL method within several "label-and-go" zero-handling workflows. For complete details on the use and execution of this protocol, please refer to Gelles et al. (2019).

Identifiants

DOI: 10.1016/j.xpro.2020.100034 PMID: 32793892 PMC: PMC7423019
pubmed: 32793892
doi: 10.1016/j.xpro.2020.100034
pmc: PMC7423019
mid: NIHMS1612596
pii:
doi:

Substances chimiques

Fluorescent Dyes 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Subventions

Organisme : NIAAA NIH HHS
ID : F31 AA024681
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA206005
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA157740
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA237264
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA196521
Pays : United States

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Auteurs

Jesse D Gelles (JD)

Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, New York, NY 10029, USA.
The Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, New York, NY 10029, USA.
Technical Contact.

Jerry E Chipuk (JE)

Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, New York, NY 10029, USA.
Department of Dermatology, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, New York, NY 10029, USA.
The Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, New York, NY 10029, USA.
The Diabetes, Obesity, and Metabolism Institute, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, New York, NY 10029, USA.
The Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, New York, NY 10029, USA.
Lead Contact.

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Classifications MeSH

Recherchemedicale.com › Phénomènes physiologiques cellulaires › Mort cellulaire › High-Throughput Cell Death Assays with...
Recherchemedicale.com › Techniques d'investigation › Techniques cytologiques › High-Throughput Cell Death Assays with...
Recherchemedicale.com › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Indicateurs et réactifs › Luminescents › Colorants fluorescents › High-Throughput Cell Death Assays with...
Recherchemedicale.com › Phénomènes chimiques › Phénomènes biochimiques › Cinétique › High-Throughput Cell Death Assays with...