Molecular cloning, sequence analysis, and tissue distribution of marmoset monoamine oxidases A and B.

The common marmoset (Callithrix jacchus), a New World primate, is currently attracting much attention as a nonhuman primate model for pharmacological and pharmacokinetic studies in preclinical research. In this study, we newly isolated the cDNAs of marmoset monoamine oxidase A (MAO-A) and MAO-B from liver and brain, respectively. MAO-A and MAO-B cDNAs, respectively, contained open reading frames of 527 and 520 amino acids and were approximately 92% and 95% identical to their human orthologs. Marmoset MAOs were phylogenetically closer to primate MAOs, including human MAOs, than to pig, dog, or rodent MAOs. The genomic and gene structures of marmoset MAOs were similar to those of humans. Among the five marmoset tissue types analyzed, the expression levels of MAO-A mRNA were relatively abundant in lung, liver, kidney, and small intestine, whereas the expression levels of MAO-B mRNA were relatively abundant in brain, liver, kidney, and small intestine; these tissue distributions are similar to those of human MAOs. These results suggest that MAO-A and MAO-B are similar at a molecular level in marmosets and humans.

Copyright © 2020 The Japanese Society for the Study of Xenobiotics. Published by Elsevier Ltd. All rights reserved.

Declaration of competing interest The authors declare that there are no conflicts of interest.