Cell blocking: An enhancement of foodborne pathogen detection by fluorescent signals of recombinant yeasts.
Fluorescent proteins
Foodborne pathogen detection
Salmonella sp.
Shigella sp.
Yeast vacuoles
cell blocking
Journal
Analytical biochemistry
ISSN: 1096-0309
Titre abrégé: Anal Biochem
Pays: United States
ID NLM: 0370535
Informations de publication
Date de publication:
01 10 2020
01 10 2020
Historique:
received:
31
03
2020
revised:
04
06
2020
accepted:
29
06
2020
pubmed:
7
8
2020
medline:
10
2
2021
entrez:
7
8
2020
Statut:
ppublish
Résumé
Cell blocking (CB) technique has been widely applied in many studies since the last century. In our research, this technique was mostly used to study the enhancement of the vacuolar response-based system that could detect Shigella sp. and Salmonella sp. investigated in previous studies. The recombinant yeast cells were blocked by mixing with agarose gel on a 96-wells plate, then storing this plate in -80 °C before using. The optimal conditions for the new system, such as agarose concentration, maximum storage time, were also established. Finally, the efficiency of the vacuolar response-based system was improved, and this system could be used as a portable detector for the foodborne pathogen.
Identifiants
pubmed: 32755600
pii: S0003-2697(20)30388-2
doi: 10.1016/j.ab.2020.113856
pii:
doi:
Substances chimiques
Fluorescent Dyes
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
113856Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.