The effects of phospholipids and fatty acids on the oligomer formation of NAP-22.
Acidic phospholipid
Lysophospholipid
NAP-22
Neuron
PUFA
Journal
Neuroscience letters
ISSN: 1872-7972
Titre abrégé: Neurosci Lett
Pays: Ireland
ID NLM: 7600130
Informations de publication
Date de publication:
25 09 2020
25 09 2020
Historique:
received:
03
07
2020
revised:
20
07
2020
accepted:
30
07
2020
pubmed:
5
8
2020
medline:
1
5
2021
entrez:
5
8
2020
Statut:
ppublish
Résumé
Recovery of various signal transduction molecules in the detergent-resistant membrane microdomain (DRM) fraction suggests the importance of this region in cellular functions. NAP-22 (also called BASP1 or CAP-23) is a neuron-enriched calmodulin-binding protein and one of the major proteins in the DRM fraction of the neuronal cell membrane. Previous studies showed tight binding activity of NAP-22 to acidic membrane lipids and the self-interaction of NAP-22, i.e., oligomerization. In this study, the effect of various phospholipids, lysophospholipids and fatty acids on the oligomerization of NAP-22 was studied through SDS-PAGE after chemical cross-linking and electron microscopic observation. High molecular mass oligomers were detected by SDS-PAGE after incubation in solutions containing over 20 mM NaCl at pH 6.5-8.5, even in the absence of lipid addition, and the addition of Ca
Identifiants
pubmed: 32750402
pii: S0304-3940(20)30558-9
doi: 10.1016/j.neulet.2020.135288
pii:
doi:
Substances chimiques
BASP1 protein, human
0
Calmodulin
0
Calmodulin-Binding Proteins
0
Fatty Acids
0
Membrane Proteins
0
Nerve Tissue Proteins
0
Phospholipids
0
Repressor Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
135288Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.