Quantitative Determination of Glymphatic Flow Using Spectrophotofluorometry.

Animals Brain / diagnostic imaging Cerebrospinal Fluid Glymphatic System / diagnostic imaging Image Processing, Computer-Assisted Mice Spectrometry, Fluorescence Spectrophotometry
Cerebrospinal fluid Fluorescent tracer Glymphatic system Spectrophotofluorometry

Journal

Neuroscience bulletin
ISSN: 1995-8218
Titre abrégé: Neurosci Bull
Pays: Singapore
ID NLM: 101256850

Informations de publication

Date de publication:
Dec 2020
Historique:
received: 14 02 2020
accepted: 06 05 2020
pubmed: 28 7 2020
medline: 29 7 2021
entrez: 26 7 2020
Statut: ppublish

Résumé

Following intrathecal injection of fluorescent tracers, ex vivo imaging of brain vibratome slices has been widely used to study the glymphatic system in the rodent brain. Tracer penetration into the brain is usually quantified by image-processing, even though this approach requires much time and manual operation. Here, we illustrate a simple protocol for the quantitative determination of glymphatic activity using spectrophotofluorometry. At specific time-points following intracisternal or intrastriatal injection of fluorescent tracers, certain brain regions and the spinal cord were harvested and tracers were extracted from the tissue. The intensity of tracers was analyzed spectrophotometrically and their concentrations were quantified from standard curves. Using this approach, the regional and dynamic delivery of subarachnoid CSF tracers into the brain parenchyma was assessed, and the clearance of tracers from the brain was also determined. Furthermore, the impairment of glymphatic influx in the brains of old mice was confirmed using our approach. Our method is more accurate and efficient than the imaging approach in terms of the quantitative determination of glymphatic activity, and this will be useful in preclinical studies.

Identifiants

DOI: 10.1007/s12264-020-00548-w PMID: 32710307 PMC: PMC7719142
pubmed: 32710307
doi: 10.1007/s12264-020-00548-w
pii: 10.1007/s12264-020-00548-w
pmc: PMC7719142
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1524-1537

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Auteurs

Yu Zhang (Y)

Department of Physiology and Neurobiology, Medical College of Soochow University, Suzhou, 215123, China.

Jian Song (J)

Department of Physiology and Neurobiology, Medical College of Soochow University, Suzhou, 215123, China.

Xu-Zhong He (XZ)

Department of Physiology and Neurobiology, Medical College of Soochow University, Suzhou, 215123, China.

Jian Xiong (J)

Institute of Medical Biotechnology, Suzhou Vocational Health College, Suzhou, 215009, China.

Rong Xue (R)

Department of Physiology and Neurobiology, Medical College of Soochow University, Suzhou, 215123, China.

Jia-Hao Ge (JH)

Department of Physiology and Neurobiology, Medical College of Soochow University, Suzhou, 215123, China.

Shi-Yu Lu (SY)

Department of Physiology and Neurobiology, Medical College of Soochow University, Suzhou, 215123, China.

Die Hu (D)

Department of Physiology and Neurobiology, Medical College of Soochow University, Suzhou, 215123, China.

Guo-Xing Zhang (GX)

Department of Physiology and Neurobiology, Medical College of Soochow University, Suzhou, 215123, China.

Guang-Yin Xu (GY)

Institute of Neuroscience, Soochow University, Suzhou, 215123, China. guangyinxu@suda.edu.cn.
ORCID: 0000-0002-5465-5892

Lin-Hui Wang (LH)

Department of Physiology and Neurobiology, Medical College of Soochow University, Suzhou, 215123, China. wanglinhui@suda.edu.cn.

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Classifications MeSH

Recherchemedicale.com › Eucaryotes › Animaux › Quantitative Determination of Glymphatic Flow...
Recherchemedicale.com › Système nerveux › Système nerveux central › Encéphale › Quantitative Determination of Glymphatic Flow...
Recherchemedicale.com › Liquides et sécrétions biologiques › Liquides biologiques › Liquide extracellulaire › Liquide cérébrospinal › Quantitative Determination of Glymphatic Flow...
Recherchemedicale.com › Systèmes sanguin et immunitaire › Système immunitaire › Système lymphatique › Système glymphatique › Quantitative Determination of Glymphatic Flow...
Recherchemedicale.com › Sciences de l'information › Méthodologies informatiques › Traitement d'image par ordinateur › Quantitative Determination of Glymphatic Flow...
Recherchemedicale.com › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Quantitative Determination of Glymphatic Flow...
Recherchemedicale.com › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Spectrométrie de fluorescence › Quantitative Determination of Glymphatic Flow...
Recherchemedicale.com › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Spectrophotométrie › Quantitative Determination of Glymphatic Flow...